Rat proximal NHE3 adapts to chronic acid-base disorders but not to chronic changes in dietary NaCl intake

American Journal of Physiology. Renal Physiology
Dominique EladariR Chambrey

Abstract

In the proximal tubule, the apical Na(+)/H(+) exchanger identified as NHE3 mediates most NaCl and NaHCO(3) absorption. The purpose of this study was to analyze the long-term regulation of NHE3 during alkalosis induced by dietary NaHCO(3) loading and changes in NaCl intake. Sprague-Dawley rats exposed to a low-NaCl, high-NaCl, or NaHCO(3) diet for 6 days were studied. Renal cortical apical membrane vesicles (AMV) were prepared from treated and normal rats. Na(+)/H(+) exchange was assayed as the initial rate of (22)Na(+) uptake in the presence of an outward H(+) gradient. (22)Na(+) uptake measured in the presence of high-dose 5-(N-ethyl-N-isopropyl) amiloride was not different among models. Changes in NaCl intake did not affect NHE3 activity, whereas NaHCO(3) loading inhibited (22)Na(+) uptake by 30%. AMV NHE3 protein abundance assessed by Western blot analysis was unaffected during changes in NaCl intake. During NaHCO(3) loading, NHE3 protein abundance was decreased by 65%. We conclude that proximal NHE3 adapts to chronic metabolic acid-base disorders but not to changes in dietary NaCl intake.

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Citations

Oct 17, 2007·Annual Review of Pharmacology and Toxicology·Vishal S VaidyaJoseph V Bonventre
May 16, 2008·American Journal of Physiology. Renal Physiology·Aaron KulickWilliam J Welch
Jul 26, 2008·American Journal of Physiology. Renal Physiology·Li E YangAlicia A McDonough
Feb 8, 2005·European Journal of Nutrition·Heidrun Kiwull-SchönePeter Kiwull
Dec 2, 2010·Cell and Tissue Research·Eleni Roussa
Nov 4, 2008·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Anne BlanchardMichel Azizi
Dec 12, 2012·Biochimica Et Biophysica Acta·Alexander G PetrenkoDominique Eladari
Jun 7, 2011·Cell Metabolism·Igor E DeyevAlexander G Petrenko
Aug 5, 2004·American Journal of Physiology. Renal Physiology·Fabienne QuentinDominique Eladari
Aug 4, 2005·American Journal of Physiology. Renal Physiology·Fiona McCullochJános Peti-Peterdi
Mar 11, 2017·Journal of the American Society of Nephrology : JASN·Yi-Min GuoLi-Ming Chen
Jan 1, 2008·Biomarker Insights·Jean-François Naud, Martine Leblanc
Aug 17, 2006·Shock·Ronald J TrofA B Johan Groeneveld
Sep 5, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Damien du CheyronMichel Paillard
May 9, 2021·The Journal of Physiology·Peder BergJens Leipziger

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