Rat TAFII31 gene is induced upon programmed cell death in differentiated PC12 cells deprived of NGF

Biochemical and Biophysical Research Communications
T AokiT Honjo

Abstract

Typical programmed cell death (PCD) requires de novo macromolecular synthesis and shares common morphological changes referred to as apoptosis. To elucidate the molecular mechanism of apoptosis, we isolated cDNA clones that are induced in differentiated PC12 cells deprived of NGF by differential display method. Among such clones, homology searches revealed that the one clone encodes the rat TATA-binding-protein-associated factor TAFII31, a component of TFIID, and a transcriptional coactivator of the p53 protein. Northern analysis of various organs in human showed one band in heart, brain, skeletal muscle and pancreas, whose size is approximately 1.1 kb which identical to that of human TAFII31 mRNA, although the size of rat human TAFII31 mRNA is approximately 2.7 kb. The deduced amino acid sequence of the rat TAFII31 was 77% identical to that of the human TAFII31. Northern analysis of various organs in adult mice showed that expression levels of TAFII31 mRNA were strong in heart but weak in spleen, although this gene is ubiquitously expressed.

Citations

May 19, 2005·Molecular and Cellular Biology·Mattia FrontiniLàszlò Tora
Mar 22, 2014·Frontiers in Oncology·Jennifer R RibeiroRichard N Freiman
Oct 26, 2000·American Journal of Medical Genetics·A M LossiC Moraine
Jul 3, 2003·The Journal of Biological Chemistry·Zheng Chen, James L Manley

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis