Rat testicular myoid cells express vasopressin receptors: receptor structure, signal transduction, and developmental regulation

Endocrinology
J HowlM Wheatley

Abstract

Detection of the neurohypophysial hormones vasopressin (AVP) and oxytocin (OT) in the testis of several species has led to the proposal that these peptides may have a physiological role in the regulation of testicular function. Therefore, we investigated whether the contractile myoid cells of rat seminiferous tubules express functional receptors for AVP or OT and, thus, constitute a target for these hormones. This study used primary cultures of purified peritubular myoid cells derived from rats both before and after puberty. By several criteria, myoid cells prepared from adult rats expressed vasopressin receptors (VPRs). We detected specific and saturable [3H]AVP binding to a single population of sites with a Kd of 7.5 nM and a binding capacity of 145 fmol/mg protein. AVP stimulated the accumulation of inositol phosphates in a dose-dependent manner with an EC50 of 1.7 nM. Cloning and sequencing of the myoid cell VPR confirmed it to be the V1a subtype of VPR. VPR expression by myoid cells is under developmental control, as the receptors are present in the adult rat, but absent before puberty. In contrast, OT receptors were not expressed at any stage of development. Peritubular myoid cells are also responsive to endothelin-1 (ET-...Continue Reading

Citations

Aug 23, 2008·Endocrinology·Catherine L Rivier
Aug 1, 1995·Molecular and Cellular Biochemistry·S A RudgeC J Kirk
Jan 25, 2011·Neuropeptides·Soon LeeCatherine Rivier
Jan 27, 2006·Human Reproduction Update·Hemlata ThackareKate Whittington
Dec 1, 1998·Endothelium : Journal of Endothelial Cell Research·J HowlM Wheatley
Mar 29, 2001·Physiological Reviews·G Gimpl, F Fahrenholz
Aug 26, 2004·Biochimica Et Biophysica Acta·Laura L WoottonFrancesco Michelangeli

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