PMID: 11933231Apr 5, 2002Paper

Ratcheting up vir gene expression in Agrobacterium tumefaciens: coiled coils in histidine kinase signal transduction

Chembiochem : a European Journal of Chemical Biology
Yulei WangDavid G Lynn

Abstract

The transmembrane histidine kinase VirA is responsible for the recognition of information from several plant-derived xenognostic signals that control gene transfer between Agrobacterium tumefaciens and its eukaryotic host. As with other histidine autokinases, VirA appears to exist as a homodimer within the inner membrane of the bacterium. In this study, we identify the putative homodimeric coiled-coil-like motifs Helix TM2 (amino acids (aa) 259-288) and Helix C (aa 293-327) within the previously assigned signal input domain. The functional importance of these coiled-coil interactions in signal-mediated VirA activation is investigated by the construction of fusion proteins with the leucine zipper domain of the transcription factor GCN4. Replacement of the membrane-spanning and periplasmic domains of VirA with the GCN4 leucine zipper gave functional proteins with increased signal-induced vir gene expression. When the GCN4 fusion was used to conformationally bias the interface of the Helix C coiled coil, constitutively active chimeras were created. The activity of these constructs was dependent on the interface of the Helix C coiled coil, and a ratchet model is proposed in which VirA activation is achieved by signal-induced switch...Continue Reading

References

Jan 1, 1992·Annual Review of Genetics·J S Parkinson, E C Kofoid
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K LeeA N Binns
Mar 1, 1992·Microbiological Reviews·S C Winans
Sep 1, 1988·Journal of Bacteriology·S C WinansE W Nester
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M D ChiltonE W Nester
Sep 15, 1994·Structure·W G Scott, B L Stoddard
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Q PanE W Nester
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Sep 1, 1993·Journal of Bacteriology·S C TurkP J Hooykaas
Jun 28, 1993·FEBS Letters·S A Benner, D L Gerloff
Jan 22, 1998·Current Opinion in Structural Biology·M M McEvoy, F W Dahlquist
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·M SinghA G Cochran
Jun 22, 1999·Chemistry & Biology·M C Pirrung
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N ShimodaY Machida

❮ Previous
Next ❯

Citations

May 20, 2006·Annual Review of Cell and Developmental Biology·Colleen A McCullen, Andrew N Binns
Jul 31, 2004·Annual Review of Phytopathology·Andrew G PalmerDavid G Lynn
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Anja Brencic, Stephen C Winans
Jun 19, 2007·Journal of Bacteriology·Rong Gao, David G Lynn
Dec 10, 2015·Plant Molecular Biology·Eva Czarnecka-VernerWilliam B Gurley
Nov 18, 2015·Proteins·E Nihal KorkmazIvan Rayment
Oct 30, 2004·Chembiochem : a European Journal of Chemical Biology·Aindrila MukhopadhyayDavid G Lynn
Jul 6, 2010·Proteins·Martin SchwalbeSteven M Pascal
Nov 14, 2012·Biochimie·Maja MalkowskaLucjan Wyrwicz
Aug 28, 2015·Chembiochem : a European Journal of Chemical Biology·Fang FangDavid G Lynn
Jun 20, 2020·ACS Synthetic Biology·Kimberly A KowallisW Seth Childers
Apr 20, 2021·Current Opinion in Microbiology·Michael J Collins, William Seth Childers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved