PMID: 8609626Mar 29, 1996Paper

Rate and selectively of synapsis of res recombination sites by Tn3 resolvase

Journal of Molecular Biology
M A WatsonW M Stark

Abstract

Site-specific recombination catalysed by Tn3 resolvase requires the formation of an intermediate synaptic complex containing two res recombination sites and several resolvase subunits. Synaptic complexes were observed directly by chemical crosslinking of resolvase subunits followed by agarose gel electrophoresis. The highest yield of synaptic complex was from a "standard" substrate, a supercoiled plasmid with res sites in direct repeat, but complexes were also made between sites in inverted repeat, or in nicked or linear molecules, or in separate molecules. The substrate selectivity for synapsis is less stringent than for recombination; thus recombination selectivity is dependent on steps after synapsis. The stability of the synapse after its formation might be a key factor, since unproductive synapses are less stable than productive ones. In a standard substrate, synapsis is fast relative to the rate of recombination. Crosslinking in active reaction mixtures yields synaptic complexes derived from both the substrate and the catenane recombination product. Although catalysis of strand exchange is at binding site I of res, a pair of isolated site 1's do not synapse, whereas a synaptic complex is formed from a plasmid carrying two...Continue Reading

Citations

Jun 5, 2002·The Journal of Biological Chemistry·Marek NapieralaRobert D Wells
Apr 9, 2005·Molecular Microbiology·Sally J RowlandW Marshall Stark
Nov 19, 2008·Nucleic Acids Research·Femi J OlorunnijiW Marshall Stark
Nov 19, 2013·PLoS Genetics·Rudra P SahaRasika M Harshey
Jun 8, 2006·Annual Review of Biochemistry·Nigel D F GrindleyPhoebe A Rice
Jan 7, 2021·Molecular Microbiology·Sally-J RowlandW Marshall Stark
Aug 29, 1997·Journal of Molecular Biology·Z Wang, P Dröge
Jun 8, 2002·Journal of Molecular Biology·J Lesley BrownMartin R Boocock
Jun 25, 2015·Microbiology Spectrum·W Marshall Stark
Jun 25, 2015·Microbiology Spectrum·Phoebe A Rice
Oct 5, 2001·Molecular Cell·G J SarkisN D Grindley

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