PMID: 6160577Oct 1, 1980Paper

Rate-limiting steps in RNA chain initiation

Proceedings of the National Academy of Sciences of the United States of America
W R McClure

Abstract

Promoter-specific lags in the approach to the steady-state rate of abortive initiation were observed when Escherichia coli RNA polymerase was added to initiate the reaction. The lag times were related to the time required for free enzyme and free promoter to combine and isomerize into a functionally active complex. The lag times measured for several bacteriophage and bacterial promoters differed widely (10 sec to several minutes) and in most cases corresponded to the rate-limiting step in the initiation process. The unique advantage in using the abortive initiation reaction to measure the lags was that the binding and isomerization steps in a simple two-state model could be quantitated separately. The separation of the contributions of both steps was effected by deriving an equation to describe the rate of formation of the active binary complex. Results from experiments based on the theory showed a linear relationship between the observed lag times and the reciprocal enzyme concentration. The slope and intercept of the equation yielded quantitative estimates of the binding and isomerization steps in initiation. The analysis was applied to the bacteriophage T7 A2 and D promoters to show the bases for the differences in in vitro ...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Mar 15, 1977·European Journal of Biochemistry·P H SeeburgH Schaller
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Johnsrud
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A von Gabain, H Bujard
Jul 1, 1977·Journal of Molecular Biology·D Kennell, H Riezman
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·N M Maizels
Jan 1, 1974·Annual Review of Biochemistry·M J Chamberlin
Jan 1, 1972·Biochimie·J L Darlix, P Fromageot
Dec 1, 1967·European Journal of Biochemistry·G WalterE Fuchs

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Citations

Sep 17, 1982·Molecular and Cellular Biochemistry·C W Wu, N Tweedy
Jul 1, 1993·World Journal of Microbiology & Biotechnology·M AldeaA Tormo
Sep 13, 2006·Bulletin of Mathematical Biology·Marc R Roussel, Rui Zhu
Jan 20, 1992·Journal of Molecular Biology·F W Whipple, A L Sonenshein
Jun 8, 2002·Journal of Molecular Biology·Ronald S Johnson, Rebecca E Chester
Sep 11, 2002·Journal of Molecular Biology·Scott E KoleskyE Peter Geiduschek
Dec 25, 2012·Nature Structural & Molecular Biology·Feng WangEric C Greene
Mar 21, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Marko Djordjevic
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Guo-Qing TangSmita S Patel
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D K Hawley, W R McClure
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J E Stefano, J D Gralla
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G K AckersM A Shea
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M C Shih, G N Gussin
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M C Shih, G N Gussin
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S M StirdivantL Bogorad
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M HerbertH Buc
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B NuezM Salas
Apr 14, 2009·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Andre S RibeiroOlli Yli-Harja
Jan 11, 1984·Nucleic Acids Research·M E MulliganW R McClure

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