Rate of incision of N-acetyl-2-aminofluorene and N-2-aminofluorene adducts by UvrABC nuclease is adduct- and sequence-specific: comparison of the rates of UvrABC nuclease incision and protein-DNA complex formation

Biochemistry
O MekhovichL J Romano

Abstract

The UvrABC nuclease, the nucleotide excision repair complex from Escherichia coli, is able to incise a variety of types of DNA damage and the repair efficiency of this enzyme complex appears to be influenced by the structure of the damage and the sequence context within which the damage is positioned. In order to better establish these relationships, we have constructed two DNA sequences each containing a site-specifically positioned N-2-aminofluorene (AF) or N-acetyl-2-aminofluorene (AAF) adduct and have determined both the kinetics of UvrABC nuclease incision and the kinetics of UvrABC nuclease-substrate complex formation. It is well established that these two adducts induce very different structures in the DNA and that these structures also depend on the sequence context. We have found that the rate of incision of both AAF- and AF-DNA adducts is significantly faster when they are positioned in the mutation hotspot NarI sequence (5-GGCG*CC-3') than when located in a normal or non-NarI sequence (5'-GATG*ATA-3') and that the rate of incision for AAF-DNA adducts is faster that for AF adducts in both sequences. Most siginificantly, we find that the rate of UvrB and UvrBC-substrate complex formation correlates with the rate of Uvr...Continue Reading

References

Dec 1, 1986·Journal of Biomolecular Structure & Dynamics·B E Hingerty, S Broyde
Aug 20, 1985·Journal of Molecular Biology·A SancarM S Tang
Nov 9, 1972·Biochimica Et Biophysica Acta·A Markovitz
Jan 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B Van Houten, A Snowden
Jan 1, 1996·Annual Review of Biochemistry·A Sancar

❮ Previous
Next ❯

Citations

Jun 8, 2001·The International Journal of Biochemistry & Cell Biology·F MengH Zhou
Nov 18, 2005·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Bongsup P Cho
Feb 2, 2006·Nucleic Acids Research·Srinivasa Rao MeneniBongsup P Cho
Jan 18, 2007·Chemical Research in Toxicology·Srinivasarao MeneniBongsup P Cho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.