Rates and product identification for trenbolone acetate metabolite biotransformation under aerobic conditions

Environmental Toxicology and Chemistry
Emily A ColeEdward P Kolodziej

Abstract

Trenbolone acetate metabolites are endocrine-active contaminants discharged into the aquatic environment in runoff from agricultural fields, rangelands, and concentrated animal feeding operations. To investigate the environmental fate of these compounds and their biotransformation mechanisms, the authors used inocula from a variety of different water sources and dosed biologically active microcosms with approximately 1400 ng/L of trenbolone acetate metabolites, including 17β-trenbolone, trendione, and 17α-trenbolone. To investigate aerobic biotransformation rates and interconversions between known trenbolone acetate metabolites, gas chromatography-tandem mass spectrometry was used to measure concentrations and assess product distributions as a function of time. High-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to characterize novel transformation products and potential transformation pathways. Kinetic analysis yields observed half-lives of approximately 0.9 d, 1.3 d, and 2.2 d for 17β-trenbolone, trendione, and 17α-trenbolone, respectively, at 20 °C, although colder conditions increased half-lives to 8.5 d and biphasic transformation was observed. Relative to reported faster attenuation rates in...Continue Reading

References

Jul 1, 1981·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J PottierI P Reynolds
Mar 5, 2004·Environmental Health Perspectives·Ana M SotoCarlos Sonnenschein
May 25, 2006·Environmental Science & Technology·Kathleen M JensenGerald T Ankley
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Karen A KiddRobert W Flick
Dec 7, 2007·Environment International·S J KhanN J Ashbolt
May 27, 2008·British Journal of Pharmacology·A T Kicman
Jun 13, 2008·Environmental Science & Technology·Bushra KhanStephen A Sassman
Aug 1, 2008·Chemosphere·Yanping Zhang, John L Zhou
Sep 10, 2008·Journal of Agricultural and Food Chemistry·Richeng XuanQiquan Wang
Dec 17, 2009·Environmental Science & Technology·Ramon LavadoDaniel Schlenk
Nov 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Masae HorinouchiToshiaki Kudo
May 31, 2011·Mutation Research·Melanie BoettcherThomas Braunbeck
Mar 13, 2012·Environmental Science & Technology·Jackson P WebsterEdward P Kolodziej
Apr 24, 2012·Environmental Science & Technology·Wei ZhengScott A Bradford
Oct 23, 2012·Chemosphere·Michael L MashtareLinda S Lee
Nov 21, 2012·Environmental Science & Technology·Shen QuDavid M Cwiertny
Dec 12, 2012·Scientific Reports·Diana A StavrevaGordon L Hager
Apr 20, 2013·Environmental Science & Technology·Edward P KolodziejDavid M Cwiertny
May 28, 2013·Environmental Science & Technology·Michael L MashtareRonald F Turco
Mar 7, 2014·Environmental Science & Technology·Gerrad D JonesEdward P Kolodziej

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