Rates of protein synthesis by hepatocytes isolated from rats of various ages

Journal of Cellular Physiology
G A RiccaA Richardson

Abstract

The rate of total protein synthesis in isolated hepatocytes was determined. The incorporation of L-[3H]valine into protein is linear for at least two hours of incubation and is affected by the concentration of amino acids in the medium. Uptake of valine by hepatocytes from 1.5- and 18-month-old rats was identical and appears to occur by simple passive diffusion. Within five minutes, the specific activities of the intracellular and extracellular valine pools are equivalent. The specific activities of these pools are saturated by 1.6mM valine and remain constant for 60 minutes of incubation. The rates of protein synthesis by hepatocytes from 1- to 2-month-old rats is 96.8 pmoles of valine per minute per milligram protein. This is comparable to rates of protein synthesis reported for perfused liver and liver in vivo and is approximately 64% higher than the rate of protein synthesis by hepatocytes from 18-month-old rats.

References

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Citations

Sep 1, 1979·Mechanisms of Ageing and Development·J J ConiglioA Richardson
Jul 1, 1987·Mechanisms of Ageing and Development·B J MerryD F Goldspink
Jun 1, 1992·Mechanisms of Ageing and Development·G FerlandB Tuchweber
Dec 1, 1979·Experimental Aging Research·R W ViskupR Penniall
Aug 1, 1983·Biological Reviews of the Cambridge Philosophical Society·S C Makrides
Sep 1, 1980·Journal of Neurochemistry·B E DwyerC G Wasterlain
Feb 14, 1984·Biochemical and Biophysical Research Communications·M M Plesko, A Richardson
Nov 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W Ward, A Richardson
Nov 1, 1989·Journal of Cellular Physiology·J A ButlerA Richardson
Dec 1, 1985·Journal of Cellular Physiology·W G RickettsA Richardson
Aug 1, 1981·Biulleten' eksperimental'noĭ biologii i meditsiny·G N Pluzhnikova, E S Ianakopulu
Jan 1, 1980·Annals of the New York Academy of Sciences·P O SeglenA Poli
Apr 1, 1984·Acta Pharmacologica Et Toxicologica·A BessesenJ Mørland
Jan 29, 1981·Biochimica Et Biophysica Acta·J HardwickA Richardson
Jul 1, 1979·Archives of Biochemistry and Biophysics·T CastleA Richardson

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