Abstract
Human melanoma cells (from biopsies and culture) express sialyl-Lewis(x) and sialyl Lewis(a), the ligands for ECAM. These ligands may facilitate tumor progression and metastasis in human cancers. To test whether the antibodies to these ligands inhibit tumor progression, IgG and IgM responses to sLe(x) and sLe(a) were induced in C57BL/6j mice (n = 76) by immunization with human melanoma cells, with or without adjuvants (BCG, MPL, KLH). Control mice were treated with saline or BCG. Tumor growth and antigen expression were monitored after challenge with B16 mouse melanoma cells expressing sLe(x), sLe(a) and the ganglioside GM3. Tumor growth was reduced in mice immunized with BCG alone or cells with BCG or MPL, while tumors in mice receiving cells without adjuvants grew larger than in the control. Augmentation of IgM titers to sLe(x) and GM3 after immunization with BCG, or with cells with BCG or MPL correlated with retarded tumor growth, while increased IgG titers to sLe(x) significantly correlated with aggressive tumor growth in mice immunized with cells without adjuvants. SLe(x), sLe(a) and GM3 were expressed in tumors from mice treated with saline or BCG. SLe(x) expression, in particular, was lost in tumors growing in mice immun...Continue Reading
References
Jan 1, 1978·Advances in Cancer Research·R W Baldwin, M V Pimm
Mar 1, 1976·British Journal of Cancer·H B HewittA S Walder
Oct 1, 1992·Annals of Surgery·D L MortonR Elashoff
Apr 1, 1991·Journal of Leukocyte Biology·Y F ChenA G Johnson
Feb 1, 1991·Journal of Immunotherapy·G T ElliottY D Kuo
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C V HardingE R Unanue
Nov 1, 1990·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D BerdM J Mastrangelo
May 1, 1988·Infection and Immunity·P J BakerJ A Rudbach
Mar 10, 1994·Journal of Immunological Methods·M H RavindranathM C Graves
Jul 1, 1994·Leukemia Research·S PulczynskiO M Jensen
Jun 1, 1995·Immunological Reviews·P O Livingston
Jan 1, 1995·Cancer·M K WallackA A Bartolucci
Jan 1, 1995·Infection and Immunity·K R MyersB Golding
Dec 1, 1994·Journal of Autoimmunity·M H RavindranathR F Irie
Jul 1, 1994·Clinical Immunology and Immunopathology·L A Manson
Jul 15, 1994·Experientia·M H RavindranathD L Morton
May 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P O LivingstonG Ritter
Jun 1, 1994·Infection and Immunity·P J BakerR J Cotter
Jun 1, 1993·Journal of Autoimmunity·M L FreimerD B Drachman
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R T Prehn
Jan 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M KirkwoodR H Blum
Sep 1, 1996·Annals of Surgical Oncology·R C JonesD L Morton
Oct 16, 1996·Journal of Immunological Methods·M H RavindranathD L Morton
Mar 1, 1997·Anti-cancer Drugs·M H RavindranathD L Morton
May 1, 1997·Cancer·M H RavindranathD L Morton
Citations
Mar 14, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Pei-Yu LiangShan-Ji Ou
Jul 15, 1999·Nature Biotechnology·T Kieber-EmmonsZ Steplewski
Apr 22, 1999·Hybridoma·J QiuT Kieber-Emmons
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christopher T CampbellJeffrey C Gildersleeve
Dec 9, 2016·Journal of Immunoassay & Immunochemistry·Ahad KhalilnezhadDavar Amani
Aug 26, 1998·Pigment Cell Research·J NakanoM Muto
Mar 30, 2004·The Journal of Biological Chemistry·Wei ZouHarold J Jennings
Jan 22, 2010·Expert Opinion on Biological Therapy·Mayrel LabradaLuis E Fernández
Jan 7, 2015·Journal of Asian Natural Products Research·Jian-Wei ChenHong Wang
Apr 30, 2016·Scientific Reports·Beata UjvariThomas Madsen
Oct 19, 1999·Microbiology and Immunology·N Okada, H Okada
Jul 27, 2001·Melanoma Research·A CarrL E Fernandez
Feb 9, 2005·DNA and Cell Biology·Yue-Jin WenThomas Kieber-Emmons
Nov 2, 1999·Micron : the International Research and Review Journal for Microscopy·J R Harris, J Markl
May 16, 2012·ACS Chemical Biology·Go SugiartoXi Chen
Oct 4, 2011·Vaccine·Jamie Heimburg-MolinaroKate Rittenhouse-Olson