Ratiometric bioassay and visualization of dopamine β-hydroxylase in brain cells utilizing a nanohybrid fluorescence probe

Analytica Chimica Acta
Niloufar AminHaidar Saify Nabiabad

Abstract

Dopamine β-hydroxylase (DBH) is involved in various neuronal transmission processes in the brain. Due to the severe diseases caused by abnormity levels of such important enzyme in human serum, sensitive and rapid detection of DBH at early stages is crucial, particularly for clinical analysis. Herein, we developed optical sensors for DBH that include the following: (i) a ratiometric fluorescence sensor that hybridizes the bovine serum albumin (BSA)-gold nanoclusters (BSA-AuNCs) and nitrogen doped carbon dots (N-CDs). The sensor proved to be highly selective and sensitive, achieving a linear range of 0.02-0.16 μg mL-1 and a limit of detection of 4.0 ng mL-1. In the presence of DBH, the fluorescence intensity of BSA-AuNCs (λem = 615 nm) was remarkably quenched by DBH serving as a reporter signal, whereas the N-CDs fluorescence intensity at 440 nm was almost kept unchanged serving as a reference signal. The developed ratiometric sensor is capable of demonstrating a color change from pink to violet and blue with a gradual increase in DBH concentration, which is discernible by the naked-eye. A test strip is prepared for semi-quantitative assay and convenient use. Intriguingly, by taking advantage of the inter-AuNCs aggregation in the...Continue Reading

Citations

Sep 10, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nesma El-Sayed, Marc Schneider
May 22, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Niloufar AminTayyebeh Madrakian

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