Ratiometric biosensor array for multiplexed detection of microRNAs based on electrochemiluminescence coupled with cyclic voltammetry

Biosensors & Bioelectronics
Xiaobin FengQianli Jiang

Abstract

A novel multiplexed ratiometric biosensor array was fabricated on a homemade screen-printed carbon electrode (SPCE) for near-simultaneous detection of microRNA (miRNA)-21 and miRNA-141 based on electrochemiluminescence (ECL) coupled with cyclic voltammetry (CV) method. In the detection system, the ECL signal tags (Ru-SiO2@PLL-Au) were fabricated using poly-l-lysine (PLL) as bridging agent and co-reactant to connect Ru-SiO2 (Ru(bpy)3(2+)-doped silica) and gold nanoparticles (Au NPs), which were respectively modified on two spatial resolved working electrodes (WE1 and WE2) of SPCE. Then the ferrocene (Fc)-labeled hairpin DNA (Fc-HDNA1 and Fc-HDNA2) as CV signal tags and ECL quenching material were immobilized on Ru-SiO2@PLL-Au. Upon miRNA-21 and miRNA-141 adding, the target miRNAs could hybridize with corresponding Fc-HDNA, which could lead to Fc away from Ru-SiO2@PLL-Au. Such conformational changes could recover the ECL of Ru-SiO2@PLL-Au and decreased the CV current of Fc, respectively. This "signal-on" of ECL and "signal-off" of CV were employed for dual-signal ratiometric readout. With the help of a multiplexed switch, two dual-signals from WE1 and WE2 were used for multiplexed detection of miRNA-21 and miRNA-141 down to 6.3 a...Continue Reading

Citations

Dec 14, 2016·Analytical Chemistry·Lingling LiJun-Jie Zhu
Oct 17, 2017·Analytical and Bioanalytical Chemistry·Despina P KalogianniTheodore K Christopoulos
Feb 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nan HaoKun Wang
Jul 12, 2017·Biosensors & Bioelectronics·Mohsen MohammadniaeiJeong-Woo Choi
Aug 3, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Qiumei FengPo Wang

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