Rational design of a water-soluble NIR AIEgen, and its application in ultrafast wash-free cellular imaging and photodynamic cancer cell ablation

Chemical Science
Dong WangBen Zhong Tang

Abstract

The synthesis of water-soluble near-infrared (NIR)-emissive fluorescent molecules with aggregation-induced emission (AIE) characteristics and theranostic functions is highly desirable but remains challenging. In this work, we designed and readily prepared for the first time such a molecule with AIE features, good water-solubility and intense emission in the NIR region. This AIE luminogen (AIEgen) is able to specifically "light up" the cell membrane without the involvement of a washing procedure. Interestingly, the staining process can be performed by simply shaking the culture with cells at room temperature for only a few seconds after the addition of the AIEgen, indicating an ultrafast and easy-to-operate staining protocol. This is the first fluorescent "light-up" probe for cell-imaging that allows the combination of a short staining period (at the second-level) with a wash-free process. Additionally, the presented AIEgen has also been developed to serve as an excellent phototherapeutic agent for high efficiency generation of reactive oxygen species (ROS) upon visible light irradiation, which allows its effective application in the photodynamic ablation of cancer cells, demonstrating its dual role as an imaging and phototherap...Continue Reading

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Citations

Feb 12, 2019·Chemistry : a European Journal·M Carmen Ortega-LiebanaJesus Santamaria
Apr 9, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Tsutomu Ishi-IYoshinori Hagiwara
Jul 16, 2019·Biomaterials Science·Feng YinBen Zhong Tang
Aug 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mengyin XuTao Yi
Oct 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuyao GuanZhigang Xie
Jan 8, 2020·Organic & Biomolecular Chemistry·Yuandong ZhangZhigang Xie
Aug 14, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Xiaofan TongZhigang Xie
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hugo A L FilipeLuís M S Loura
Aug 8, 2020·Chemical Communications : Chem Comm·You-Hui ZhangJong Seung Kim
Oct 13, 2018·Chemical Science·Yuan GuBen Zhong Tang
Oct 30, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Lijuan LiZhigang Xie
Dec 15, 2020·Organic & Biomolecular Chemistry·Mykhaylo A PotopnykJuozas Vidas Grazulevicius
Jan 6, 2021·Journal of Drug Targeting·Rashmi Kumari, Dhanya Sunil
Dec 29, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fei Meng
May 21, 2020·Angewandte Chemie·Dingyuan YanBen Zhong Tang
Feb 16, 2021·Journal of Drug Targeting·Rashmi Kumari, Dhanya Sunil
Nov 11, 2020·Pharmaceutics·Artur Y PrilepskiiVladimir V Vinogradov
Nov 17, 2020·Chemical Society Reviews·Guangxue FengDan Ding
Feb 25, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fei ZhangBin Liu
Apr 21, 2021·ACS Applied Materials & Interfaces·Peilin GuJianlei Shen

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Methods Mentioned

BETA
light scattering
biosensing
fluorescence imaging
Fluorescence
dynamic
transmission electron microscopy
flow cytometry

Software Mentioned

ZEN
CellMask

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