Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function

The International Journal of Biochemistry & Cell Biology
Fritha HennessyGregory L Blatch

Abstract

Prokaryotic DnaJ and DnaK, homologous to the eukaryotic 40 and 70kDa heat shock proteins (Hsp40 and Hsp70) respectively, play an important role as molecular chaperones in assisted protein folding under both normal and stressed conditions. DnaJ-like proteins are defined by the presence of a 70 amino acid domain termed the J domain, similar to the initial 73 amino acids of the Escherichia coli protein DnaJ. The J domain comprises four alpha-helices and a loop region containing the invariant tripeptide of histidine, proline and aspartic acid (HPD motif). This motif and Helix II have been shown previously to be important for the interaction with partner Hsp70s. Conserved amino acid residues present in the J domain were identified, and substitutions of these residues were performed to examine their effect on the in vivo functioning of the J domain of Agrobacterium tumefaciens DnaJ. Three conserved, charged residues, and three conserved, hydrophobic residues, in addition to the HPD motif, were shown to be important for the correct functioning of A. tumefaciens DnaJ. These included Arg26 located on Helix II, Arg63 and Asp59 located on Helix IV, Tyr7 and Leu10 located on Helix I, and Leu57 located on Helix III. This study has identifie...Continue Reading

References

Nov 15, 1979·Analytical Biochemistry·P A LanzettaO A Candia
Apr 1, 1992·Trends in Biochemical Sciences·P BorkB Bukau
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Mar 1, 1990·Journal of Molecular Graphics·G Vriend
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K LiberekM Zylicz
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C Bardwell, E A Craig
Mar 15, 1996·The Journal of Biological Chemistry·B Banecki, M Zylicz
Jul 12, 1996·Journal of Molecular Biology·M PellecchiaK Wüthrich
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·W L Kelley, C Georgopoulos
Mar 15, 1997·The Biochemical Journal·L H Chamberlain, R D Burgoyne
Feb 12, 1998·The Journal of Biological Chemistry·L H Chamberlain, R D Burgoyne
May 19, 1998·Cell Stress & Chaperones·M E Cheetham, A J Caplan
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K GreeneS J Landry
Jun 30, 1998·Trends in Biochemical Sciences·W L Kelley
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C S GässlerB Bukau
May 5, 1999·Current Biology : CB·W L Kelley
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T LaufenB Bukau
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A J MacarioE Conway de Macario
Apr 11, 2000·Molecular and Cellular Biology·J L Johnson, E A Craig
Jul 13, 2000·Journal of Molecular Biology·M Martinez-YamoutH J Dyson
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sheara W FewellJeffrey L Brodsky
Aug 17, 2002·The Journal of Biological Chemistry·Christoph J KluckMatthias P Mayer
Mar 26, 2003·The Journal of Biological Chemistry·Wanjiang Han, Philipp Christen
Apr 30, 2003·Biochemistry·Pernilla Wittung-StafshedeSamuel J Landry

❮ Previous
Next ❯

Citations

Aug 17, 2014·Plant Physiology and Biochemistry : PPB·Zongliang XiaJianjun Liu
Nov 28, 2012·Cellular and Molecular Life Sciences : CMLS·Yinong Zong, Rongsheng Jin
Aug 5, 2008·The International Journal of Biochemistry & Cell Biology·Eva-Rachele PesceGregory L Blatch
Jan 8, 2013·The Plant Journal : for Cell and Molecular Biology·Jian-Zhong Liu, Steven A Whitham
Oct 10, 2013·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Özlem Tastan BishopGregory Lloyd Blatch
Dec 7, 2007·The International Journal of Biochemistry & Cell Biology·Aileen BoshoffGregory L Blatch
Jan 24, 2007·The International Journal of Biochemistry & Cell Biology·W S NicollG L Blatch
Sep 14, 2013·Journal of Biomolecular Structure & Dynamics·Rowan HatherleyOzlem Tastan Bishop
Sep 20, 2011·Cancer Letters·Jason N SterrenbergAdrienne L Edkins
Apr 13, 2007·The International Journal of Biochemistry & Cell Biology·M BothaG L Blatch
Oct 30, 2016·Biochemical and Biophysical Research Communications·Yahui WuWuming Xiao
Jul 1, 2005·Protein Science : a Publication of the Protein Society·Fritha HennessyGregory L Blatch
Dec 5, 2020·International Journal of Biological Macromolecules·Hongjie FengHeqin Zhu
Oct 4, 2005·Protein Expression and Purification·William S NicollGregory L Blatch
Aug 29, 2006·Neurobiology of Disease·Zahra FayaziParsa Kazemi-Esfarjani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.