Rationally engineered synthetic coculture for improved biomass and product formation

PloS One
Suvi SantalaVille Santala

Abstract

In microbial ecosystems, bacteria are dependent on dynamic interspecific interactions related to carbon and energy flow. Substrates and end-metabolites are rapidly converted to other compounds, which protects the community from high concentrations of inhibitory molecules. In biotechnological applications, pure cultures are preferred because of the more straight-forward metabolic engineering and bioprocess control. However, the accumulation of unwanted side products can limit the cell growth and process efficiency. In this study, a rationally engineered coculture with a carbon channeling system was constructed using two well-characterized model strains Escherichia coli K12 and Acinetobacter baylyi ADP1. The directed carbon flow resulted in efficient acetate removal, and the coculture showed symbiotic nature in terms of substrate utilization and growth. Recombinant protein production was used as a proof-of-principle example to demonstrate the coculture utility and the effects on product formation. As a result, the biomass and recombinant protein titers of E. coli were enhanced in both minimal and rich medium simple batch cocultures. Finally, harnessing both the strains to the production resulted in enhanced recombinant protein ti...Continue Reading

References

Apr 5, 2001·Current Opinion in Biotechnology·J R Swartz
Jan 23, 2004·Journal of Immunological Methods·Ville Santala, Urpo Lamminmäki
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Alan J Wolfe
May 19, 2005·Biotechnology Advances·Joseph Shiloach, Rephael Fass
Sep 13, 2005·Current Opinion in Biotechnology·Lee R LyndMark Laser
Sep 15, 2006·Trends in Biotechnology·Mark A Eiteman, Elliot Altman
Aug 2, 2007·Journal of Industrial Microbiology & Biotechnology·Marjan De MeyErick Vandamme
Jan 22, 2008·ACS Chemical Biology·Jay D Keasling
Feb 29, 2008·Journal of Biological Engineering·Mark A EitemanElliot Altman
Mar 6, 2008·Molecular Systems Biology·Véronique de BerardinisJean Weissenbach
Aug 5, 2008·Trends in Biotechnology·Katie BrennerFrances H Arnold
Nov 11, 2008·Current Opinion in Biotechnology·Sung Kuk LeeJay D Keasling
Apr 22, 2009·Journal of the American Chemical Society·Travis S BayerChristopher A Voigt
May 23, 2009·Nature Reviews. Molecular Cell Biology·Priscilla E M Purnick, Ron Weiss
Oct 1, 2010·Biotechnology and Bioengineering·Timothy J Hanly, Michael A Henson
May 20, 2011·Microbial Cell Factories·Suvi SantalaVille Santala
Oct 4, 2011·Microbial Cell Factories·Suvi SantalaVille Santala
Oct 22, 2011·Journal of Biotechnology·Hans C BernsteinRoss P Carlson
Oct 25, 2011·Applied and Environmental Microbiology·Charles Daniel MurinIchiro Matsumura
Mar 16, 2013·Chemical Reviews·Christine A Rabinovitch-DeereShota Atsumi
Feb 17, 2012·ACS Synthetic Biology·Suvi SantalaVille Santala
May 16, 2014·Journal of the Royal Society, Interface·Lisa GoersKaren M Polizzi
Jun 6, 2014·ACS Synthetic Biology·Suvi SantalaVille Santala

❮ Previous
Next ❯

Citations

Jul 25, 2015·Current Opinion in Microbiology·Olga Ponomarova, Kiran Raosaheb Patil
Jun 4, 2016·The ISME Journal·Tobias GroßkopfOrkun S Soyer
Nov 21, 2016·Journal of Industrial Microbiology & Biotechnology·Matti KannistoVille Santala
Mar 24, 2017·FEMS Microbiology Letters·Tapio LehtinenSuvi Santala
Feb 10, 2018·Microbial Cell Factories·Tapio LehtinenVille Santala
Jan 31, 2019·Life·Jai A Denton, Chaitanya S Gokhale
Apr 14, 2020·PLoS Computational Biology·Marco MauriEugenio Cinquemani
May 18, 2016·FEMS Microbiology Letters·Ville SantalaSuvi Santala
Jul 11, 2018·Biotechnology for Biofuels·Milla SalmelaRahul Mangayil
Aug 29, 2018·Biotechnology for Biofuels·Tapio LehtinenVille Santala
Jan 12, 2021·Computational and Structural Biotechnology Journal·Beatriz García-JiménezJuan Nogales
Feb 1, 2021·Current Opinion in Biotechnology·Martijn DienderDiana Z Sousa
Mar 27, 2021·Essays in Biochemistry·Suvi Santala, Ville Santala
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hoang Thi Hong AnhAndrew S Ball
Feb 13, 2018·ACS Sustainable Chemistry & Engineering·Martijn DienderDiana Z Sousa
Jul 16, 2019·New Biotechnology·Rahul MangayilVille Santala

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knock-out
PCR
Fluorescence

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.