Rats bred for enhanced apomorphine susceptibility have elevated tyrosine hydroxylase mRNA and dopamine D2-receptor binding sites in nigrostriatal and tuberoinfundibular dopamine systems

Brain Research
N Y RotsE R de Kloet

Abstract

From a Wistar population two rat lines were generated using as criterion the behavioral response to the dopamine agonist apomorphine. Rats of the apomorphine-susceptible (apo-sus) line revealed a vigorous gnawing response to apomorphine administration while the other rat line, the apomorphine-unsusceptible (apo-unsus) line, was selected for lack of response to the drug. In the present study using the 12th and 13th generation of these genetically selected lines, we have investigated whether this difference in apomorphine responsiveness was correlated with changes in dopamine neurochemistry. Therefore, we measured tyrosine hydroxylase (TH), the rate limiting enzyme in dopamine synthesis, as well as dopamine D1 and D2 receptor mRNA levels in discrete brain regions by in situ hybridization. Dopamine (D2/D3) receptor binding was assessed with [125I]iodosulpride in a membrane binding assay and by quantitative autoradiography on tissue sections. [3H]SCH 23390 was used to analyze D1 receptor binding. Apo-sus rats displayed significantly higher TH mRNA levels in the A9 cell group of the substantia nigra pars compacta and in the A12 cell group of the arcuate nucleus. No difference was found in the A10 cell group of the VTA and the A6 cel...Continue Reading

References

Jan 1, 1990·Brain Research Bulletin·A R CoolsB Ellenbroek
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E M SternbergR L Wilder
Dec 22, 1988·Nature·J R BunzowO Civelli
Jan 1, 1972·Neuroendocrinology·W H GispenE R de Kloet
Apr 6, 1981·Brain Research·J A SeversonC E Finch
Apr 6, 1993·European Journal of Pharmacology·B ParsonsJ Javitch
Jan 1, 1995·Behavioural Pharmacology·W.D. EssmanI. Lucky

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Citations

Jun 1, 1996·Cellular and Molecular Neurobiology·E R de KloetA R Cools
Jun 29, 2004·Psychoneuroendocrinology·Marc A T TeunisAnnemieke Kavelaars
Feb 13, 2001·Pharmacology, Biochemistry, and Behavior·F SluyterA R Cools
Sep 23, 2000·Neuroscience Research·N M de BruinB A Ellenbroek
Sep 20, 1996·Annals of the New York Academy of Sciences·E R De KloetM R Kruk
Jan 22, 2009·British Journal of Pharmacology·Kwok Ho Christopher ChoyMaarten van den Buuse
Jan 30, 2002·Brain Research·Sabine GermeyerUrsula Havemann-Reinecke
Oct 29, 2005·European Journal of Pharmacology·Bart A EllenbroekAlexander R Cools
Oct 7, 2008·Behavioural Brain Research·Dubravka HranilovicYanyan Wang
Oct 1, 2016·Scientific Reports·Martijn M SeltenGerard J M Martens
Sep 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marc A T TeunisCobi J Heijnen
Jun 17, 1998·Endocrine Reviews·E R De KloetM Joëls
Feb 11, 2021·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·D A MaasG J M Martens

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