Rats surviving injurious mechanical ventilation show reversible pulmonary, vascular and inflammatory changes

Intensive Care Medicine
Nicolás NinAndrés Esteban

Abstract

To describe the time course of the changes in pulmonary and vascular function, and systemic inflammation induced by injurious mechanical ventilation. Experimental study in an animal model of ventilator-induced lung injury. Animal research laboratory. Anesthetized male adult Sprague-Dawley rats were ventilated with VT 9 ml/kg and PEEP 5 cmH2O, or VT 35 ml/kg and zero PEEP for 1 h, and were killed. Other rats received ventilation for 1 h with high VT, to observe survival (n=36), or to be monitored and killed at different points in time (24, 72 and 168 h; n=7 in each group). Blood samples for measuring biochemical parameters were obtained. Post-mortem, a bronchoalveolar lavage (BAL) was performed, the aorta and pulmonary microvessels were isolated to examine ex-vivo vascular responses and pulmonary slices were examined (light microscopy). Mortality in rats ventilated with high VT was 19 of 36 (54%). Mechanical ventilation was associated with hypotension, hypoxaemia and membrane hyaline formation. AST, ALT, IL-6, MIP-2 serum and BAL fluid concentrations, as well as VEGF BAL fluid concentration, were increased in rats ventilated with high VT. Lung injury score was elevated. Aortic vascular responses to acetylcholine and norepinephri...Continue Reading

References

Nov 1, 1975·Journal of Applied Physiology·S L SelingerN C Staub
May 1, 1994·American Journal of Respiratory and Critical Care Medicine·J G MuscedereA S Slutsky
Mar 1, 1997·The Journal of Clinical Investigation·L TremblayA S Slutsky
Jan 28, 1998·American Journal of Respiratory and Critical Care Medicine·D Dreyfuss, G Saumon
May 29, 1999·Circulation Research·J PanS Ogawa
Jul 3, 1999·American Journal of Respiratory and Critical Care Medicine·D ChiumelloA S Slutsky
Jun 29, 2000·JAMA : the Journal of the American Medical Association·V M RanieriA S Slutsky
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·H D HeldS Uhlig
Nov 24, 2001·American Journal of Respiratory and Critical Care Medicine·D R ThickettA B Millar
Mar 29, 2003·American Journal of Respiratory and Critical Care Medicine·Won-Il ChoiCharles A Hales
May 10, 2003·American Journal of Respiratory Cell and Molecular Biology·Marcella M MascarenhasDeborah A Quinn
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Oct 12, 2004·Journal of Applied Physiology·Marco MuraMingyao Liu

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Citations

Jan 9, 2008·Intensive Care Medicine·Joan R Badia, Niall D Ferguson
Mar 21, 2012·Critical Care : the Official Journal of the Critical Care Forum·Adrián González-López, Guillermo M Albaiceta
Mar 30, 2010·Critical Care : the Official Journal of the Critical Care Forum·Rosanna VaschettoFrans B Plötz
Apr 11, 2009·Intensive Care Medicine·Leticia Martínez-CaroAndrés Esteban
Jul 27, 2010·Experimental Lung Research·Patricia Rose ChessCarl J Johnston
Dec 28, 2011·European Journal of Pharmacology·Li AnSen-Yang Yu
Mar 22, 2013·Critical Care Medicine·Carmen MenendezFrancisco Perez-Vizcaino
Aug 30, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Alba Chacon-CabreraEsther Barreiro
Nov 21, 2013·Anesthesiology·José L Izquierdo-GarcíaJosé A Lorente
Sep 29, 2011·Anesthesiology·Paolo Pelosi, Patricia R M Rocco
Nov 1, 2016·Cell and Tissue Research·Sean E GillRuud A W Veldhuizen
Jul 8, 2016·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Fernando Fonseca Dos ReisBruno Valle Pinheiro
Jul 12, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adrián González-LópezGuillermo M Albaiceta
Aug 8, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thaís Fernanda FazzaMaycon Moura Reboredo
Jun 9, 2016·Minerva pediatrica·Jinjie HuangJun Wu
Aug 19, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kai SuGuochang Hu

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