raw Functions through JNK signaling and cadherin-based adhesion to regulate Drosophila gonad morphogenesis.

Developmental Biology
Jennifer C JemcMark Van Doren

Abstract

To form a gonad, germ cells (GCs) and somatic gonadal precursor cells (SGPs) must migrate to the correct location in the developing embryo and establish the cell-cell interactions necessary to create proper gonad architecture. During gonad morphogenesis, SGPs send out cellular extensions to ensheath the individual GCs and promote their development. We have identified mutations in the raw gene that result in a failure of the SGPs to ensheath the GCs, leading to defects in GC development. Using genetic analysis and gene expression studies, we find that Raw negatively regulates JNK signaling during gonad morphogenesis, and increased JNK signaling is sufficient to cause ensheathment defects. In particular, Raw functions upstream of the Drosophila Jun-related transcription factor to regulate its subcellular localization. Since JNK signaling regulates cell adhesion during the morphogenesis of many tissues, we examined the relationship between raw and the genes encoding Drosophila E-cadherin and β-catenin, which function together in cell adhesion. We find that loss of DE-cadherin strongly enhances the raw mutant gonad phenotype, while increasing DE-cadherin function rescues this phenotype. Further, loss of raw results in mislocalizati...Continue Reading

References

Dec 22, 1998·Mechanisms of Development·P Rørth
Jan 29, 2003·The Journal of Cell Biology·Anne PacqueletPernille Rorth
Aug 16, 2003·Developmental Cell·Tony J DeFalcoMark Van Doren
Oct 28, 2003·Nature Cell Biology·Michelle A LiDorothea Godt
Aug 16, 2005·Current Opinion in Cell Biology·Jack Lilien, Janne Balsamo
Mar 29, 2006·Developmental Biology·Stéphanie Le Bras, Mark Van Doren
Feb 20, 2007·Biochimica Et Biophysica Acta·Gary L Johnson, Kazuhiro Nakamura
Apr 24, 2008·Genetics·Katherine L BatesAnthea Letsou
Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Brian E Richardson, Ruth Lehmann
Jan 12, 2010·Cellular Signalling·Eitan Shaulian
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Geert Berx, Frans van Roy
May 14, 2010·American Journal of Physiology. Cell Physiology·O BermudezC Gimond
Jul 8, 2011·Genesis : the Journal of Genetics and Development·Jennifer C Jemc

❮ Previous
Next ❯

Citations

Oct 31, 2012·Genesis : the Journal of Genetics and Development·Luis Daniel Ríos-Barrera, Juan Rafael Riesgo-Escovar
Jun 15, 2014·Developmental Biology·Ratna TripathyAndrew D Renault
Feb 25, 2015·PLoS Genetics·Luis Daniel Ríos-BarreraJuan Rafael Riesgo-Escovar
Mar 9, 2017·Nature Communications·Jun ZhouMichael Boutros
Oct 6, 2018·Genesis : the Journal of Genetics and Development·Victoria R HansJennifer C Jemc
Apr 22, 2018·G3 : Genes - Genomes - Genetics·Abeer HassanAdi Salzberg
Sep 4, 2020·Biology Open·Marta PortelaSergio Casas-Tintó
May 31, 2018·PloS One·Diana LuongJennifer C Jemc

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.