PMID: 11337282May 19, 2001Paper

RB activation and repression of C-MYC transcription precede apoptosis of human prostate epithelial cells

Urology
X Zhao, M L Day

Abstract

The retinoblastoma gene (Rb) encodes a transcriptional regulatory protein that functions in the regulation of cellular growth, differentiation, and survival. Many of the target genes of Rb with respect to growth regulation and differentiation have been identified. However, the identities of the Rb target genes involved in the regulation of cell survival and cell death (apoptosis) are unknown. We sought to determine whether the c-myc oncogene, a known target of Rb activity in cell cycle control, is also recruited in an apoptotic pathway uniquely regulated by Rb in prostate epithelial cells. We previously described a cell culture model to study the apoptosis of prostate cancer cells in which the human prostate cancer line, LNCaP, will undergo apoptosis after inducible expression and activation of the alpha isozyme of protein kinase C (PKC) or after exposure to low concentrations of the PKC activator TPA. Rb protein and c-myc mRNA and protein were evaluated in the Rb+/+ LNCaP and in the Rb-/- DU145 prostate cancer cells. TPA-induced apoptosis in LNCaP cells was preceded by the rapid depletion of c-myc mRNA. The DU145 cultures were resistant to TPA-induced apoptosis and the c-myc mRNA levels remained elevated. The examination of Rb...Continue Reading

Citations

Mar 24, 2006·Apoptosis : an International Journal on Programmed Cell Death·N GodefroyJ-L Vayssière
Apr 6, 2006·Omics : a Journal of Integrative Biology·Stewart ChoHilmi Ozcelik
Sep 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhuo ZhangRuiwen Zhang
Mar 18, 2008·Alcoholism, Clinical and Experimental Research·Peter Kuhn, Dipak K Sarkar
Jul 20, 2010·Biochemical and Biophysical Research Communications·Nathalie Le FlochJean-Luc Vayssière
Aug 6, 2004·Biochemical Pharmacology·Giuliana CassinelliFranco Zunino
May 13, 2004·Human Molecular Genetics·Pamela L ParisHerman van Dekken
Jul 29, 2006·The Journal of Biological Chemistry·Yin-Choy ChuanAmilcar Flores-Morales
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Louis RagoliaJohn K Maesaka

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis