RBBP6 expressional effects on cell proliferation and apoptosis in breast cancer cell lines with distinct p53 statuses

Cancer Management and Research
Lesetja R MotadiPontsho Moela

Abstract

Breast cancer is the most common malignancy amongst women and has a higher incidence rate than lung cancer. Its tumor progression partially results from inactivation of p53 which is caused by overexpression of ubiquitous regulatory proteins possessing p53-binding domain. RBBP6 is regarded as one of the ubiquitous proteins because of its RING finger-like domain which enables it to possess E3 ligase activity. Thus, it has become a potential target in cancer treatment as it is highly expressed in various malignancies including cancer. However, it is not clearly defined whether the effect of RBBP6 on cell growth and apoptosis is cell line-dependent, more especially in breast cancer cell lines that have distinct p53 expression profiles. This study aims at evaluating the effects of RBBP6 on cell growth and apoptosis in breast cancer cell lines with different p53 expressions. Following the analysis at mRNA and protein levels in breast cancer tissue, RBBP6 expression was successfully manipulated using gene silencing and protein overexpression techniques in MCF-7 and MDA-MB-231 cell lines. The cells were co-treated with siRBBP6 and anticancer agents following apoptosis detection, which was confirmed by caspase 3/7 activity and quantific...Continue Reading

Citations

Oct 28, 2019·Wiley Interdisciplinary Reviews. RNA·Michael Robert Murphy, Frida Esther Kleiman
Nov 6, 2020·Cancer Management and Research·Mpho MosweuPontsho Moela

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Methods Mentioned

BETA
light microscopy
scraping
Protein Assay
flow cytometry
confocal microscopy
PCR
electrophoresis
transfection
transfections
flow

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