RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response

Signal Transduction and Targeted Therapy
Zezhong LiuShibo Jiang

Abstract

The pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed serious threats to global health and economy, thus calling for the development of safe and effective vaccines. The receptor-binding domain (RBD) in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2 (ACE2) receptor. It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines. Here, we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG, as an immunopotentiator, elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections. The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus (SARSr-CoV). Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge. These results suggest that SARS-Co...Continue Reading

References

Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxian HeShibo Jiang
May 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxian HeShibo Jiang
Dec 8, 2007·Nature Reviews. Immunology·Falk Nimmerjahn, Jeffrey V Ravetch
Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Aug 25, 2011·Current Opinion in Biotechnology·Roland E Kontermann
Apr 29, 2014·Expert Review of Vaccines·Naru ZhangLanying Du
Jun 28, 2016·Nature Reviews. Microbiology·Emmie de WitVincent J Munster
Feb 13, 2020·Cellular & Molecular Immunology·Shuai XiaLu Lu
Feb 23, 2020·Lancet·Shibo JiangDeyin Guo
Mar 4, 2020·Nature Microbiology·UNKNOWN Coronaviridae Study Group of the International Committee on Taxonomy of Viruses
Apr 1, 2020·The New England Journal of Medicine·Nicole LurieJane Halton
Apr 9, 2020·Frontiers in Microbiology·Ning WangLanying Du
Apr 11, 2020·Nature Reviews. Drug Discovery·Tung Thanh LeStephen Mayhew
May 8, 2020·Nature·Linlin BaoChuan Qin
May 15, 2020·Cell Host & Microbe·Dennis R Burton, Laura M Walker
May 21, 2020·Nature Communications·Trevor R F SmithKate E Broderick
Jun 9, 2020·Lancet·Asher Mullard
Jun 22, 2020·Lancet· The Lancet
Jul 3, 2020·Cell Research·Bao-Zhong ZhangJian-Dong Huang
Jul 8, 2020·The Journal of Clinical Investigation·Yanqun WangJincun Zhao
Jul 15, 2020·The New England Journal of Medicine·Lisa A JacksonUNKNOWN mRNA-1273 Study Group
Jul 31, 2020·Nature·Neeltje van DoremalenVincent J Munster
Aug 14, 2020·Nature·Mark J MulliganKathrin U Jansen
Aug 17, 2020·Cell·Na-Na ZhangCheng-Feng Qin

❮ Previous
Next ❯

Citations

Mar 25, 2021·Cell Research·Shan SuShibo Jiang
Apr 16, 2021·Expert Review of Vaccines·Mohnad AbdallaXinyi Jiang
Apr 28, 2021·Current Opinion in Virology·Chung-Young Lee, Anice C Lowen
May 11, 2021·Frontiers in Molecular Biosciences·Long Min, Qiu Sun
Jun 25, 2021·International Immunopharmacology·Hamid MotamediRamin Abiri

❮ Previous
Next ❯

Datasets Mentioned

BETA
QHD43416.1

Methods Mentioned

BETA
ELISA
transgenic
chip
glycosylation
transfection
Assay
fluorescence microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.