RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing

Nature Medicine
Wei GuoMichael Gotthardt

Abstract

Alternative splicing has a major role in cardiac adaptive responses, as exemplified by the isoform switch of the sarcomeric protein titin, which adjusts ventricular filling. By positional cloning using a previously characterized rat strain with altered titin mRNA splicing, we identified a loss-of-function mutation in the gene encoding RNA binding motif protein 20 (Rbm20) as the underlying cause of pathological titin isoform expression. The phenotype of Rbm20-deficient rats resembled the pathology seen in individuals with dilated cardiomyopathy caused by RBM20 mutations. Deep sequencing of the human and rat cardiac transcriptome revealed an RBM20-dependent regulation of alternative splicing. In addition to titin (TTN), we identified a set of 30 genes with conserved splicing regulation between humans and rats. This network is enriched for genes that have previously been linked to cardiomyopathy, ion homeostasis and sarcomere biology. Our studies emphasize the key role of post-transcriptional regulation in cardiac function and provide mechanistic insights into the pathogenesis of human heart failure.

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Related Concepts

Cardiomyopathy, Familial Idiopathic
Pathogenic Aspects
Sarcomeres
Pathogenesis
Post-Transcriptional Regulation
RBM20 protein, rat
LIM Domain Protein
Ion Homeostasis
RBM20 gene
TTN gene

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