Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex

Cell Metabolism
Milena VukoticMarkus Deckers

Abstract

The terminal enzyme of the mitochondrial respiratory chain, cytochrome oxidase, transfers electrons to molecular oxygen, generating water. Within the inner mitochondrial membrane, cytochrome oxidase assembles into supercomplexes, together with other respiratory chain complexes, forming so-called respirasomes. Little is known about how these higher oligomeric structures are attained. Here we report on Rcf1 and Rcf2 as cytochrome oxidase subunits in S. cerevisiae. While Rcf2 is specific to yeast, Rcf1 is a conserved subunit with two human orthologs, RCF1a and RCF1b. Rcf1 is required for growth in hypoxia and complex assembly of subunits Cox13 and Rcf2, as well as for the oligomerization of a subclass of cytochrome oxidase complexes into respirasomes. Our analyses reveal that the cytochrome oxidase of mitochondria displays intrinsic heterogeneity with regard to its subunit composition and that distinct forms of respirasomes can be formed by complex variants.

References

Apr 25, 2000·The EMBO Journal·H Schägger, K Pfeiffer
Oct 26, 2000·Trends in Biochemical Sciences·S Raha, B H Robinson
May 31, 2001·Methods in Cell Biology·M T RyanN Pfanner
Oct 2, 2001·American Journal of Medical Genetics·E A Shoubridge
Feb 2, 2002·The EMBO Journal·Patrick PaumardJean Velours
May 21, 2003·Accounts of Chemical Research·Heather S Carr, Dennis R Winge
Jun 27, 2003·The New England Journal of Medicine·Salvatore DiMauro, Eric A Schon
Aug 14, 2003·Current Neurology and Neuroscience Reports·Massimo Zeviani, Antonella Spinazzola
Oct 17, 2003·The Journal of Biological Chemistry·Kathy PfeifferHermann Schägger
Jun 9, 2006·American Journal of Physiology. Cell Physiology·Flavia FontanesiAntoni Barrientos
Jul 22, 2006·Journal of Molecular Biology·Matthew McKenzieMichael T Ryan
Nov 23, 2006·Current Biology : CB·Martin van der LaanNikolaus Pfanner
Sep 1, 2007·Molecular & Cellular Proteomics : MCP·Jörg ReindersChris Meisinger
Sep 21, 2007·The EMBO Journal·David U MickPeter Rehling
May 10, 2008·IUBMB Life·Flavia FontanesiAntoni Barrientos
Oct 8, 2008·Journal of Bioenergetics and Biomembranes·Rosemary A Stuart
Nov 26, 2008·Molecular Cell·Rebeca Acín-PérezJose Antonio Enriquez
Jan 27, 2009·Biochimica Et Biophysica Acta·Ilka Wittig, Hermann Schägger
Jun 17, 2009·The Journal of Cell Biology·Regina RablAndreas S Reichert
Jul 29, 2010·The Journal of Biological Chemistry·Yudong WangJerry Vockley
Sep 30, 2010·The Journal of Cell Biology·David U MickPeter Rehling
Nov 6, 2010·Journal of Microscopy·C A WurmS Jakobs
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·David U MickPeter Rehling
May 7, 2011·Annual Review of Biophysics·Shinya YoshikawaKyoko Shinzawa-Itoh
Sep 13, 2011·The EMBO Journal·Thorsten AlthoffWerner Kühlbrandt

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Citations

Sep 26, 2012·Biotechnology Letters·M Isabel González SisoM Esperanza Cerdán
Sep 18, 2012·Molecular & Cellular Proteomics : MCP·F-Nora VögtleChris Meisinger
Apr 16, 2013·Antioxidants & Redox Signaling·Evelina MaranzanaMaria Luisa Genova
Sep 4, 2012·Antioxidants & Redox Signaling·Myriam BourensAntoni Barrientos
Jul 19, 2013·Molecular Biology of the Cell·Lena BöttingerThomas Becker
Jul 19, 2013·Journal of the Royal Society, Interface·Peter R Rich, Amandine Maréchal
Jul 3, 2013·Science·Esther Lapuente-BrunJosé Antonio Enríquez
Mar 27, 2013·Molecular and Cellular Biology·Ketki M HatleMercedes Rincon
May 16, 2012·Molecular and Cellular Biology·Dennis R Winge
Nov 14, 2013·Chemistry and Physics of Lipids·Eugenia Mileykovskaya, William Dowhan
Jan 31, 2014·Molecular Biology of the Cell·Chen-Hsien SuAlexander Tzagoloff
May 21, 2014·The Journal of Cell Biology·Markus HildenbeutelMartin Ott
Jan 7, 2016·Annual Review of Physiology·José Antonio Enríquez
Nov 8, 2015·Biochimica Et Biophysica Acta·Axel Magalon, François Alberge
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Takaharu HayashiSeiji Takashima
Jan 29, 2016·Biochimica Et Biophysica Acta·Giorgio LenazMaria Luisa Genova
Nov 15, 2012·The EMBO Journal·Werner J H KoopmanPeter H G M Willems
Apr 17, 2016·Biochimica Et Biophysica Acta·Maria LevchenkoMarkus Deckers
Dec 26, 2013·Biochimica Et Biophysica Acta·Rebeca Acin-Perez, Jose A Enriquez
Apr 20, 2016·Biochimica Et Biophysica Acta·Valentina StreckerIlka Wittig
Dec 4, 2014·Cell Metabolism·Arnaud MourierDusanka Milenkovic
Nov 5, 2013·Biochimica Et Biophysica Acta·Yuriy ChabanNatalya V Dudkina
Nov 20, 2013·Biochimica Et Biophysica Acta·Maria Luisa Genova, Giorgio Lenaz
Aug 21, 2012·Cell Metabolism·Eduardo BalsaJosé Antonio Enríquez
Feb 15, 2014·Biochemical and Biophysical Research Communications·Steffen LindertSenyon Choe
Apr 24, 2016·Free Radical Biology & Medicine·Raquel Moreno-Loshuertos, José Antonio Enríquez
Apr 1, 2016·Molecular Biology of the Cell·Bettina BarethSven Dennerlein
Jun 25, 2016·International Journal of Molecular Sciences·Dayan SunJianxin Lyu
Jul 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Camilla Rydström LundinPeter Brzezinski
Nov 5, 2016·Biochimica Et Biophysica Acta·Irina A SmirnovaPeter Brzezinski
Apr 6, 2017·Cell Metabolism·Dusanka MilenkovicJudy Hirst

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