rDNA-RFLP identification of Candida species in immunocompromised and seriously diseased patients

Canadian Journal of Microbiology
Patrícia M PintoMiriam Tendler

Abstract

PCR was used to amplify a targeted region of the ribosomal DNA of 76 Candida spp. isolates from immunocompromised and seriously diseased patients. Thirty-seven strains isolated from different anatomical sites of 11 patients infected with HIV (Vitória, ES, Brazil), 26 isolates from patients under treatment at Odilon Behrens Hospital and 13 isolates from skin and urine samples from São Marcos Clinical Analysis Laboratory (Belo Horizonte, Brazil) were scored. Fragments of rDNA were amplified using primer pairs ITS1-ITS4, for the amplification of ITS1 and ITS2 regions, including the gene for the 5.8 s subunit. Amplification resulted in fragments ranging in size from 350 to 950 bp. Amplicons were digested with eight restriction enzymes. A pattern of species-specificity among the different medically important Candida species could be identified following restriction digestion of the PCR products. Candida albicans was the species most frequently observed, except for the group of newborns under treatment at the Odilon Behrens Hospital and for the isolates from the clinical analysis laboratory. C. parapsilosis was the species most frequently observed in these two groups.

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Citations

Aug 31, 2006·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Hossein MirhendiHideyo Yamaguchi
Dec 21, 2010·Revista iberoamericana de micología·Gioconda San-Blas, Eva Burger
May 16, 2006·Revista Da Sociedade Brasileira De Medicina Tropical·Henrique Maia ValérioMaria Aparecida de Resende
Mar 15, 2005·Yeast
Apr 21, 2010·Indian Journal of Medical Microbiology·T ShokohiS Mayahi

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