Re-188 labelled antibodies

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
B A RhodesE B Harvey

Abstract

Monoclonal antibodies can be directly labelled with 188Re using a simple one-step radiolabelling kit. Using B72.3 as a model antibody, the formulation was optimized and kits were made and tested and compared to data previously reported for the same antibody labelled with other radioisotopes. Labelling with Re-188 was carried out with the eluate of a W-188/Re-188 generator from Oak Ridge National Laboratory. Fresh generator eluate was added to the pre-reduced lyophilized antibody and the mixture allowed to incubate overnight at room temperature. The radiochemical purity, immunoreactive fraction, and biodistribution in normal and LS174T tumor bearing nude mice was determined. The radiochemical purity was 88 +/- 7%, the immunoreactive fraction was 68.46 +/- 3.8%. The immunoreactive fraction was higher than any previously reported for this antibody when labelled with other radioisotopes. At 48 h, 7.9 +/- 2.4% of the injected dose per gram was found in the tumor. The biodistribution and tumor uptake of Re-188 labelled B72.3 was similar to that previously reported for Re-186 and In-111 labelled B72.3.

References

May 1, 1992·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·M M AlauddinA L Epstein
Dec 1, 1990·Clinical Nuclear Medicine·J F EaryJ L Vanderheyden
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D ColcherJ Schlom

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Citations

Feb 2, 2006·European Journal of Nuclear Medicine and Molecular Imaging·Sandrine BallotJean-Pierre Benoit
Nov 14, 2002·International Journal of Pharmaceutics·José L CrudoSilvia G de Castiglia
Feb 24, 2001·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·N Iznaga-Escobar
Jul 1, 1997·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·T R SykesA A Noujaim
Sep 6, 2000·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·J DuA R Holmberg
Mar 8, 2003·Nuclear Medicine and Biology·Chang-bin LiuDonald J Hnatowich
Nov 14, 1997·Nuclear Medicine and Biology·C J SmithW A Volkert
Nov 6, 1998·Nuclear Medicine and Biology·P F SchmidtP G Bundesen
Nov 25, 2003·Cancer Biotherapy & Radiopharmaceuticals·Jae Min Jeong, June-Key Chung
Jan 17, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Rogier LangeHarry Hendrikse
Oct 9, 2014·Journal of Zhejiang University. Science. B·Chai-Hong YeongKwan-Hoong Ng
Sep 28, 2017·BioMed Research International·Licia UccelliAlessandra Boschi
Dec 26, 2001·Chemical Reviews·W A Volkert, T J Hoffman
Oct 29, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Liang-Ting LinYi-Jang Lee

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