Re-characterization of hammerhead ribozymes as molecular tools for intermolecular RNA cleavage

Organic & Biomolecular Chemistry
Getong LiuZhuo Tang

Abstract

A new guideline for the construction of hammerhead ribozymes to achieve trans-cleavage of a single-stranded RNA molecule was developed. The sequence rule of the HHRz cleavage site was highly recommended to be "DWH" with an optimal binding arm length of 8-9nt, which diverged from the former rule of "NUX".

References

Nov 27, 1990·Biochemistry·D E RuffnerO C Uhlenbeck
Aug 13, 1987·Nature·O C Uhlenbeck
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N K VaishF Eckstein
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·J E Wedekind, D B McKay
Sep 2, 1998·Nucleic Acids Research·A R KoreF Eckstein
Feb 6, 2002·Chembiochem : a European Journal of Chemical Biology·F EcksteinK L Nakamaye
Apr 17, 2003·FEMS Microbiology Reviews·Elena Puerta-FernándezAlfredo Berzal-Herranz
Jul 19, 2003·RNA·Ronald R BreakerNarasimhan Sudarsan
Jul 26, 2003·Nature Structural Biology·Anastasia KhvorovaSumedha D Jayasena
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M BuzayanG Bruening
Aug 17, 2006·RNA·Manami Roychowdhury-Saha, Donald H Burke
Sep 23, 2006·Chembiochem : a European Journal of Chemical Biology·Rita Przybilski, Christian Hammann
Aug 6, 2010·Current Opinion in Pharmacology·Jérôme MulhbacherDaniel A Lafontaine
Nov 26, 2010·Cold Spring Harbor Perspectives in Biology·Ronald R Breaker
Jan 25, 2011·The Journal of Biological Chemistry·Randi M JimenezAndrej Lupták
Jan 31, 2012·Chemistry & Biology·John C Burnett, John J Rossi
Mar 29, 2012·RNA·Christian HammannMarcos de la Peña
Sep 23, 2014·Chemistry & Biology·Ronald R Breaker, Gerald F Joyce
Oct 21, 2015·Trends in Biochemical Sciences·Randi M JimenezAndrej Lupták
Jan 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcos de la PeñaAmelia Cervera

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