Re-Classification of Drosophila melanogaster Trichoid and Intermediate Sensilla Using Fluorescence-Guided Single Sensillum Recording

PloS One
Chun-Chieh Lin, Christopher J Potter

Abstract

Drosophila olfactory receptor neurons are found within specialized sensory hairs on antenna and maxillary palps. The linking of odorant-induced responses to olfactory neuron activities is often accomplished via Single Sensillum Recordings (SSR), in which an electrode inserted into a single sensory hair records the neuronal activities of all the neurons housed in that sensillum. The identification of the recorded sensillum requires matching the neuronal responses with known odor-response profiles. To record from specific sensilla, or to systematically screen all sensillar types, requires repetitive and semi-random SSR experiments. Here, we validate an approach in which the GAL4/UAS binary expression system is used for targeting specific sensilla for recordings. We take advantage of available OrX-Gal4 lines, in combination with recently generated strong membrane targeted GFP reporters, to guide electrophysiological recordings to GFP-labeled sensilla. We validate a full set of reagents that can be used to rapidly screen the odor-response profiles of all basiconic, intermediate, and trichoid sensilla. Fluorescence-guided SSR further revealed that two antennal trichoid sensilla types should be re-classified as intermediate sensilla....Continue Reading

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Citations

Oct 4, 2016·Nature Communications·Olena RiabininaChristopher J Potter
Jul 2, 2019·Journal of Chemical Ecology·Ian W KeeseyMarkus Knaden
Jun 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Neeraj SoniJohn R Carlson
Oct 26, 2018·Frontiers in Molecular Neuroscience·Sarah KoerteMarkus Knaden
Sep 15, 2018·Frontiers in Cellular Neuroscience·Carolina Gomez-DiazEsther Alcorta
Apr 7, 2019·Nature Communications·Ye ZhangChih-Ying Su
Mar 13, 2019·Nature Communications·Ian W KeeseyBill S Hansson
Oct 31, 2019·Chemical Senses·Shashank ChepurwarNitin Gupta

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Methods Mentioned

BETA
light microscopy
dissection
transgenic

Software Mentioned

FgSSR
Arduino
AUTOSPIKE

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