Re-evaluation of the serotypes of Serratia marcescens and separation into two schemes based on lipopolysaccharide (O) and capsular polysaccharide (K) antigens.

Microbiology
Hazel M AuckenTyrone L Pitt

Abstract

Chemical and serological analysis has revealed that many of the 29 O serotype reference strains of Serratia marcescens contain both neutral and acidic polysaccharides which correspond to LPS O antigens and capsular K antigens, respectively. New O and K antigen typing schemes have therefore been devised, based on the known chemical structures of the surface polysaccharides of the organism. These schemes were designed to allow the specific detection of these antigens on unknown strains using ELISAs. O antigens were detected using whole cells cultured in broth then autoclaved to remove capsular material, while K antigens were detected using formolized whole cells which had been cultured on glycerol agar to enhance capsule production. After testing with the 29 reference strains as well as 423 distinct clinical strains, it was apparent that different aspects of chemical structure were associated with different degrees of serological reactivity and the typing schemes were modified further to accommodate this. In general, the O antigen repeating unit structures were chemically simple with di- or trisaccharide backbones. Serological specificity was often provided solely by the presence or absence of an O-acetyl substituent, or a change...Continue Reading

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Citations

May 1, 2008·Biochemical and Biophysical Research Communications·Po-Chi SooHsin-Chih Lai
Jun 27, 2007·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yoko YanagawaTadakatsu Shimamura
Aug 30, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D S HansenH J Kolmos
Oct 17, 2017·Microbiology·Xiqian WangAidong Han
Mar 10, 2004·The Veterinary Record·J JoresA Lübke-Becker
Nov 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kenichi HaradaYasuni Nakanuma

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