Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry

Methods and Applications in Fluorescence
Nicolás I Krimer, Martin Mirenda

Abstract

In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, K SQ. We measured the absorption and the steady-state emission spectra of quinine bisulphate, QBS, solutions ranging between 1.5 × 10-5 and 1.5 × 10-1 M. From these data, we calculated the expected emission spectra, affected by re-absorption, for all QBS concentrations. For higher concentrations, the re-absorption in the excitation/detection direction reaches values up to 6% of the total emitted intensity. The K SQ of the dye was re-evaluated from the concentration dependence of the quotients between the calculated and the experimental integrated emission spectra. The obtained value, K SQ = 18.4 ± 0.1 M-1, shows no significant differences with those obtained from steady-state and average lifetimes by other authors, pointing out the diffusional nature of the self-quenching phenomenon. The present work helps clarify some ambiguous aspects concerning the photophysics of QBS, stressing that re-absorption phenomena must be considered in QBS concentrated solutio...Continue Reading

References

May 1, 1967·Analytical Biochemistry·R F Chen
Jan 28, 2011·The Journal of Physical Chemistry. B·Xu-Wei ChenJian-Hua Wang
Jul 23, 2013·Nature Protocols·Christian WürthUte Resch-Genger
May 1, 1976·Journal of Research of the National Bureau of Standards. Section A, Physics and Chemistry·J B Birks

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Citations

Oct 20, 2018·The Journal of Physical Chemistry. B·David PunihaoleRenee R Frontiera
Nov 30, 2018·Accounts of Chemical Research·Hernán B RodríguezEnrique San Román

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