Re-examination of the role of the periplasmic domain of EnvZ in sensing of osmolarity signals in Escherichia coli

Molecular Microbiology
M R Leonardo, S Forst

Abstract

In Escherichia coli, EnvZ senses changes in the osmotic conditions of the growth environment and controls the phosphorylated state of the regulatory protein, OmpR. OmpR-phosphate regulates the expression of the porin genes, ompF and ompC. To investigate the role of the periplasmic domain of EnvZ in sensing of osmolarity signals, portions of this domain were deleted. Cells containing the EnvZ mutant proteins were able to regulate normally the production of OmpF and OmpC in response to changes in osmolarity. The periplasmic domain of EnvZ was also replaced with the non-homologous periplasmic domain of the histidine kinase PhoR of Bacillus subtilis. Osmoregulation of OmpF and OmpC production in cells containing the PhoR-EnvZ hybrid protein was indistinguishable from that in cells containing wild-type EnvZ. Identical results were obtained with an envZ-pta/ack strain, which could not synthesize acetyl phosphate. Thus, acetyl phosphate was not involved in the regulation of ompF and ompC observed in this study. These results indicate that the periplasmic domain of EnvZ is not essential for sensing of osmolarity signals.

Citations

Mar 6, 1999·Current Opinion in Microbiology·P N Goudreau, A M Stock
May 8, 2002·Molecular Microbiology·Scott M WardMichael D Manson
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Thorsten MascherGottfried Unden
Aug 2, 2008·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·A O Spakov, M N Pertseva
Sep 10, 2010·Annual Review of Microbiology·Tino KrellJuan Luis Ramos
Apr 20, 2017·FEBS Letters·Eunha HwangChaejoon Cheong
Sep 2, 2006·Journal of Proteome Research·Lina WuXuanxian Peng
May 20, 2009·Mikrobiologiia·A O Shpakov
Jul 8, 2010·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Reinhard Krämer
Aug 28, 1998·Journal of Molecular Biology·C G HeadL J Kenney
Dec 1, 2004·EcoSal Plus·R Gary Sawers, David P Clark
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M C GustinK Davenport

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.