Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

International Journal of Molecular Medicine
Kang XiaoXiao-Ping Dong

Abstract

It is well known that the SMB-S15 cell line was originally established by cultures from the brains of mice affected by the Chandler scrapie strain, and this cell line may express PrPSc permanently. However, the infectivity of the S15-derived prions on experimental animals has not yet been well documented. In the present study, the cell lysates of SMB-S15 were intracerebrally inoculated into three different strains of mice, namely C57BL/6, Balb/c and CD1. Prion protein (PRNP) gene sequencing revealed the same encoded PrP proteins in the sequences of amino acids in the three strains of mice, in addition to a synonymous single nucleotide polymorphism (SNP) in CD1 mice. All infected mice developed typical experimental transmissible spongiform encephalopathies (TSEs) approximately six months post-infection. The clinical features of three infected mice were comparable. The pathogenic characteristics, such as the electrophoretic and glycosylation profiles and proteinase K (PK) resistance of PrPSc molecules, as well as the neuropathological characteristics, such as spongiform vacuolation, PrPSc deposits in cortex regions, astrogliosis and activated microglia, were also similar in all three strains of infected mice. However, PrPSc depos...Continue Reading

References

Apr 1, 1985·Cell·B OeschL E Hood
Mar 1, 1988·The Journal of General Virology·B CaugheyB Chesebro
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R K MeyerS B Prusiner
Apr 29, 1986·Biochemical and Biophysical Research Communications·J E Fleming, E L Kwak
May 1, 1987·The Journal of General Virology·R E RaceB Chesebro
Dec 4, 1984·Biochemistry·D C BoltonS B Prusiner
Oct 27, 2001·Neuropathology and Applied Neurobiology·N F McLennanJ W Ironside
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nathan R DeleaultSurachai Supattapone
Jun 23, 2007·Nature Reviews. Molecular Cell Biology·Adriano AguzziMagdalini Polymenidou
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·David W Colby, Stanley B Prusiner

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Citations

Nov 9, 2016·Emerging Microbes & Infections·Chen GaoXiao-Ping Dong
Dec 7, 2016·Journal of Molecular Neuroscience : MN·Xiao -Dong YangXiao-Ping Dong

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Methods Mentioned

BETA
PCR
electrophoresis
glycosylation

Software Mentioned

SPSS
ImageJ

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