Re-oxygenation after anoxia induces brain cell death and memory loss in the anoxia-tolerant crucian carp

The Journal of Experimental Biology
Sjannie LefevreGöran E Nilsson

Abstract

Crucian carp (Carassius carassius) survive without oxygen for several months, but it is unknown whether they are able to protect themselves from cell death normally caused by the absence, and particularly return, of oxygen. Here, we quantified cell death in brain tissue from crucian carp exposed to anoxia and re-oxygenation using the terminal deoxy-nucleotidyl transferase dUTP nick-end labelling (TUNEL) assay, and cell proliferation by immunohistochemical staining for proliferating cell nuclear antigen (PCNA) as well as PCNA mRNA expression. We also measured mRNA and protein expression of the apoptosis executer protease caspase 3, in laboratory fish exposed to anoxia and re-oxygenation and fish exposed to seasonal anoxia and re-oxygenation in their natural habitat over the year. Finally, a behavioural experiment was used to assess the ability to learn and remember how to navigate in a maze to find food, before and after exposure to anoxia and re-oxygenation. The number of TUNEL-positive cells in the telencephalon increased after 1 day of re-oxygenation following 7 days of anoxia, indicating increased cell death. However, there were no consistent changes in whole-brain expression of caspase 3 in either laboratory-exposed or natu...Continue Reading

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Citations

Jul 26, 2018·Frontiers in Genetics·Claire L RiggsJason E Podrabsky
Nov 2, 2018·The Journal of Experimental Biology·Milica Mandic, Matthew D Regan
Jul 12, 2020·The Journal of Experimental Biology·Georgina K Cox, Todd E Gillis
Jul 17, 2020·The Science of the Total Environment·Yue ZhangYuehong Li
Aug 19, 2021·PloS One·Andres Felipe ZambranoEduardo Castillo
Aug 27, 2021·Metabolites·Alexander Myrka, Leslie Buck
Dec 15, 2021·Metallomics : Integrated Biometal Science·Yue ZhangYuehong Li

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