RE1 silencing transcription factor (REST) negatively regulates ALL1-fused from chromosome 1q (AF1q) gene transcription

BMC Molecular Biology
Yuanyuan HuXiulian Sun

Abstract

ALL1-fused from chromosome 1q (AF1q), originally considered as an oncogenic factor, has been implicated in neuronal development; however, its upstream regulatory mechanisms in neural system remained elusive. Our study showed that REST (RE1 silencing transcription factor), a key transcription factor in neurodevelopment, could down-regulate the gene expression of AF1q. The promoter assay identified a neuron-restrictive silencer element at -383 to -363 bp of human AF1q promoter. Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (CHIP) confirmed the binding of REST to the NRSE in AF1q gene promoter. Additionally, the negative correlation between the expression levels of Af1q and Rest in mice neurodevelopment supported the negative regulation of AF1q by REST and the potential functions of AF1q in neurodevelopment. These results demonstrate that REST regulates AF1q gene transcription through directly binding to a NRSE at -383 to -363 bp of AF1q promoter.

References

Feb 20, 1999·Leukemia·M Busson-Le ConiatR Berger
Dec 30, 2004·The Journal of Clinical Endocrinology and Metabolism·Caroline JacquesPascal Reynier
Sep 19, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Da-Qiang LiZhi-Min Shao
Nov 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiulian SunWeihong Song
Oct 12, 2007·Breast Cancer Research and Treatment·Xin-Zhong ChangZhi-Ming Shao
Mar 31, 2010·The Journal of Cell Biology·Jun KohyamaKinichi Nakashima
Jan 22, 2011·The Journal of Biological Chemistry·Mei LuXiulian Sun
Jul 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhengliang GaoJenny Hsieh
Feb 19, 2013·Trends in Cell Biology·Sara NegriniJacopo Meldolesi
Mar 29, 2014·Nature·Tao LuBruce A Yankner
May 21, 2014·Gene Expression Patterns : GEP·Makiko YamadaAngelo Iulianella
Dec 15, 2015·Cancer Research·Andreas M HoffRolf I Skotheim

❮ Previous
Next ❯

Citations

Sep 22, 2019·Protein Expression and Purification·Nazimuddin KhanT Michael Sabo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
ChIP
pulldown
transfections

Software Mentioned

Ensemble

Related Concepts

Related Feeds

Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous disease with approximately 20,000 cases per year in the United States. AML also accounts for 15-20% of all childhood acute leukemias, while it is responsible for more than half of the leukemic deaths in these patients. Here is the latest research on this disease.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Alzheimer's Disease: Transcription

Impaired transcription is associated with the pathogenesis and progression of conditions such as Alzheimer's disease (AD). Here are the latest discoveries pertaining to transcription and AD.

AML: Role of LSD1 by CRISPR (Keystone)

Find the latest rersearrch on the ability of CRISPR-Cas9 mutagenesis to profile the interactions between lysine-specific histone demethylase 1 (LSD1) and chemical inhibitors in the context of acute myeloid leukemia (AML) here.