Reactant stationary approximation in enzyme kinetics

The Journal of Physical Chemistry. a
Sonya M Hanson, S Schnell

Abstract

In the application of the quasi-steady-state approximation, it is generally assumed that there is an initial transient during which the substrate concentration remains approximately constant while the complex concentration builds up. In this paper, we address the assumption that the substrate concentration does not change significantly during this initial transient and name it the reactant stationary approximation. For the single enzyme, single substrate reaction, the reactant stationary approximation is generally considered a sufficient condition to apply the quasi-steady-state approximation. Studying the dynamic behavior of this reaction with endogenous substrate, we show that the quasi-steady-state approximation and reactant stationary approximation are two separate approximations. We discuss the consequence of this result for the determination of reaction parameters in enzyme catalyzed reactions.

References

Aug 21, 1979·Journal of Theoretical Biology·M Schauer, R Heinrich
Mar 1, 1989·The Biochemical Journal·C T Zimmerle, C Frieden
May 17, 2000·Bulletin of Mathematical Biology·S Schnell, P K Maini
May 29, 2000·Bulletin of Mathematical Biology·S Schnell, C Mendoza
Sep 22, 2006·Systems Biology·E H Flach, S Schnell
Feb 29, 2008·The Journal of Physical Chemistry. a·Banghe LiBo Li

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Citations

Sep 3, 2010·PloS One·Srabanti Chaudhury, Oleg A Igoshin
Aug 1, 2015·Bulletin of Mathematical Biology·Richard Kollár, Katarína Šišková
Dec 3, 2013·The FEBS Journal·Ramon GrimaSantiago Schnell
Mar 27, 2015·The FEBS Journal·Maria F PintoPedro M Martins
Jul 23, 2014·Biophysical Chemistry·Mark WhiddenSantiago Schnell
Mar 15, 2015·Frontiers in Plant Science·Abir U Igamberdiev
Sep 28, 2016·Biophysical Chemistry·Wylie Stroberg, Santiago Schnell
Oct 4, 2016·Mathematical Biosciences·Wylie Stroberg, Santiago Schnell
Nov 5, 2016·Expert Opinion on Drug Discovery·Rumin Zhang, Kenny Wong
Jun 3, 2016·Physical Chemistry Chemical Physics : PCCP·Suzanne K Shoffner, Santiago Schnell
Oct 6, 2018·Bulletin of Mathematical Biology·Justin Eilertsen, Santiago Schnell
Jul 1, 2019·Mathematical Biosciences·Dimitris G Patsatzis, Dimitris A Goussis
Apr 12, 2020·Mitochondrion·Abir U Igamberdiev
Sep 28, 2018·Mathematical Biosciences·Justin EilertsenSantiago Schnell
Aug 10, 2020·Journal of Molecular Biology·Maria Filipa PintoPedro M Martins
Nov 18, 2018·Progress in Biophysics and Molecular Biology·Abir U Igamberdiev, Leszek A Kleczkowski
Aug 19, 2021·NPJ Systems Biology and Applications·Juliana Reves SzemereAlejandra C Ventura

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