Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16

Applied Microbiology and Biotechnology
Torben HoefelDirk Weuster-Botz

Abstract

Recombinant Cupriavidus necator H 16 with a novel metabolic pathway using a cobalamin-dependent mutase was exploited to produce 2-hydroxyisobutyric acid (2-HIBA) from renewable resources through microbial fermentation. 2-HIBA production capacities of different strains of C. necator H 16 deficient in the PHB synthase gene and genetically engineered to enable the production of 2-HIBA from the intracellular PHB precursor (R)-3-hydroxybutyryl-CoA were evaluated in 48 parallel milliliter-scale stirred tank bioreactors (V = 11 mL). The effects of media composition, limitations, pH, and feed rate were studied with respect to the overall process performances of the different recombinant strains. 2-HIBA production was at a maximum at nitrogen limiting conditions and if the pH was controlled between 6.8 and 7.2 under fed-batch operating conditions (intermittent fructose addition). The final concentration of 2-HIBA was 7.4 g L(-1) on a milliliter scale. Best reaction conditions identified on the milliliter scale were transferred to a laboratory-scale fed-batch process in a stirred tank bioreactor (V = 2 L). Two different process modes for the production of 2-HIBA, a single-phase and a dual-phase fermentation procedure, were evaluated and ...Continue Reading

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Citations

Dec 25, 2010·Applied Microbiology and Biotechnology·Ralf Hortsch, Dirk Weuster-Botz
Aug 15, 2013·Applied Microbiology and Biotechnology·Denise PrzybylskiRoland H Müller
Aug 31, 2013·Chemical Reviews·Adrie J J Straathof
Mar 5, 2013·Journal of Industrial Microbiology & Biotechnology·Jaciane Lutz IenczakGláucia Maria Falcão de Aragão
Dec 31, 2011·Bioresource Technology·Peter Riedlberger, Dirk Weuster-Botz
Apr 28, 2011·Biotechnology Progress·Gabi GebhardtDirk Weuster-Botz
Jan 4, 2012·Biotechnology Journal·Michael Schmidt, Dirk Weuster-Botz
Jun 28, 2015·Journal of Biotechnology·Andreas SchmidederDirk Weuster-Botz
Dec 14, 2017·Critical Reviews in Biotechnology·Matthias RabergAlexander Steinbüchel
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Juliana LebeauMichael D Lynch
May 26, 2018·World Journal of Microbiology & Biotechnology·Gurusamy Kutralam-Muniasamy, Fermín Peréz-Guevara
Jan 13, 2021·Macromolecular Rapid Communications·Hugo Fouilloux, Christophe M Thomas

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