PMID: 100425Sep 15, 1978

Reaction of 7,12-dimethylbenz(a)anthracene with DNA of fetal and maternal rat tissues in vivo

International Journal of Cancer. Journal International Du Cancer
G DoerjerP Kleihues

Abstract

Pregnant BD-IX rats (21st day of gestation) received a single IV injection (15 mg/kg) of tritiated 7,12-dimethylbenz(a)anthracene (DMBA), A DOSE KNOWN TO INduce a high incidence of nervous-system tumors in the offspring. The animals were killed 12 h later and hydrocarbon-deoxyribonucleoside products from DNA of maternal and fetal tissues were separated on Sephadex LH-20 columns eluted with a 20-100% methanol gradient. Concentrations of the major DMBA-DNA adduct varied considerably, with highest values in maternal intestine, liverand lung, followed by spleen, kidney and brain. In fetal intestine and liver, concentrations were 34% and 16% lower than in the respective maternal organs whereas the reaction with cerebral DNA was 2 1/2 times higher in fetuses than in the pregnant mother. This indicates that there is no significant placental barrier to DMBA or DMBA metabolites involved in DNA binding and that rat fetuses participate in the metabolic formation of the ultimate carcinogen.

References

Jan 1, 1977·Mutation Research·P Brookes
Jan 1, 1977·International Journal of Cancer. Journal International Du Cancer·E Huberman, L Sachs
Jun 20, 1977·Biochemical and Biophysical Research Communications·R C MoschelA Dipple
Sep 15, 1977·International Journal of Cancer. Journal International Du Cancer·W M Baird, A Dipple
Jan 1, 1978·Journal of the National Cancer Institute·D H Janss, T L Ben
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A M JeffreyK Nakanishi
May 1, 1976·Journal of the National Cancer Institute·J A KellenA L Fletch
Oct 13, 1977·Nature·P Kleihues, J Bucheler
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H W KingP Brookes
Oct 15, 1975·International Journal of Cancer. Journal International Du Cancer·M R OsborneP Brookes
Apr 1, 1974·Journal of the National Cancer Institute·N Napalkov, V A Alexandrov
Jun 1, 1974·Chemico-biological Interactions·G T BowdenR K Boutwell

Citations

Nov 1, 1980·Archives of Toxicology·H M Bolt, R J Laib
Feb 1, 1982·Photochemistry and Photobiology·R E Gibson, S M D'Ambrosio
Feb 14, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Lizette GranbergEva B Brittebo

Related Concepts

Benz(a)Anthracenes
Metabolic Biotransformation
DNA, Double-Stranded
Fetal Structures
Maternal-Fetal Exchange
Drug or Chemical Tissue Distribution
9,10-Dimethyl-1,2-benzanthracene

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