PMID: 2502392Jul 1, 1989Paper

Reaction of fluorescein isothiocyanate with an ATP-binding site on the phosphorylase kinase alpha subunit

European Journal of Biochemistry
N ZamanJ W Crabb

Abstract

Phosphorylase kinase can be labeled specifically on the alpha subunit with fluorescein 5'-isothiocyanate (FITC) which concomitantly inactivates the enzyme (T. G. Sotiroudis and S. Nikolaropoulus (1984) FEBS Lett. 176, 421-425). Labeled peptides have been purified and their primary structure has been determined. The amino acid sequence of the fluorescein-labeled tryptic peptide is Lys-Met-Gln-Asp-Gly-Tyr-Phe-Gly-Gly-Ala-Arg. The environment of this fluorescein-labeled lysine has been determined by sequencing peptides isolated from a Staphylococcus aureus V8 digest and two further cyanogen bromide fragments of the purified [14C]carboxymethylated alpha subunit. The partial sequences obtained have then been localized in the primary structure of the alpha subunit [Zander et al. (1988) Proc. Natl Acad. Sci. USA 85, 2929-2933]. Both the incorporation of the fluorescent label and enzymatic inactivation are inhibited by ATP only at pH 7.0; ADP and AMP do not protect. Kinetic analysis reveals a competition between ATP and FITC; a Ki for ATP of 728 +/- 100 microM has been determined.

References

Oct 14, 1987·Biochemical and Biophysical Research Communications·W J Ball, M L Friedman
Jul 16, 1985·Biochemical and Biophysical Research Communications·T OhtaM Yoshida
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M W KilimannL M Heilmeyer
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N F ZanderM W Kilimann
Jan 2, 1985·European Journal of Biochemistry·Z HessováL M Heilmeyer
Jan 1, 1974·Analytical Biochemistry·H P Jennissen, L M Heilmeyer
May 15, 1974·FEBS Letters·H P Jennissen, L M Heilmeyer
Jun 16, 1972·Biochimica Et Biophysica Acta·L B Clerch, F Huijing
Dec 14, 1984·Biochemical and Biophysical Research Communications·T L KirleyL K Lane

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Citations

Nov 1, 1993·Molecular and Cellular Biochemistry·L M HeilmeyerH P Jennissen
Nov 23, 1977·Biochimica Et Biophysica Acta·M A GordonA J Trevor
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·G VerebL M Heilmeyer
Sep 14, 2015·Journal of Molecular Graphics & Modelling·Jaida BegumJoseph M Hayes
Jun 1, 1994·Journal of Inorganic Biochemistry·T P Geladopoulos, T G Sotiroudis
Sep 13, 1991·Biochimica Et Biophysica Acta·L M Heilmeyer

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