PMID: 37905Aug 14, 1979

Reaction of the purple membrane with a carbodiimide

Biochimica Et Biophysica Acta
R RenthalR Parrish

Abstract

Purple membrane was reacted with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at pH 4.5 and 8.0. At pH 4.5, the reaction yields cross-linked bacteriorhodopsin. The cross-linking is inhibited by pretreatment of the membrane with papain, or by the presence of carbohydrazide or glycine ethyl ester in the reaction mixture. The product of the pH 8.0 reaction is not cross-linked, but it displays altered properties. Two measures of photochemical activity (light-induced change in proton binding (delta h) and decay of photointermediate M) show changes indicative of slowed proton uptake. The delta h is increased by ethyl dimethylaminopropylcarbodiimide. This increase is unaffected by pretreatment of the membrane with papain, and it is not reversed by NH2OH. However, the reaction is inhibited by millimolar concentrations of CaCl2. The altered delta h is not apparent in detergent-solubilized membranes. Ethyl dimethylaminopropylcarbodiimide does not appear to cause a large alteration in the membrane surface charge, as measured by Ca2+ binding. We conclude that (1) at acid pH, ethyl dimethylaminopropylcarbodiimide can be used for cross-linking or for attachment of specific probes to the C-terminal region of bacteriorhodopsin, and hence to t...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G E GerberH G Khorana
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G E GerberH G Khorana

Citations

Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C H ChangT Ebrey
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T MogiH G Khorana
Nov 1, 1984·Photochemistry and Photobiology·Y KimuraW Stoeckenius
Oct 30, 1984·Biochemical and Biophysical Research Communications·S Wu-ChouL Packer
Mar 12, 1987·Biochimica Et Biophysica Acta·R RenthalG Harris
Dec 14, 1979·Biochemical and Biophysical Research Communications·G HarrisN Robinson
Oct 3, 1980·Biochimica Et Biophysica Acta·R Renthal, B Wallace
Jul 30, 1981·Biochemical and Biophysical Research Communications·R RenthalL Villarreal
Oct 1, 1982·European Journal of Biochemistry·Y A OvchinnikovV P Skulachev
Aug 1, 1983·International Journal of Peptide and Protein Research·R Renthal

Related Concepts

Bacteriorhodopsins
Carbodiimides
Carotenoids
Halobacterium
Hydrogen-Ion Concentration
Light
Tromasin
Plasma Protein Binding Capacity
Spectrophotometry

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