PMID: 11342058May 9, 2001Paper

Reactions of soybean peroxidase and hydrogen peroxide pH 2.4-12.0, and veratryl alcohol at pH 2.4

Biochimica Et Biophysica Acta
M NissumK G Welinder

Abstract

Peroxidase from soybean seed coat (SBP) has properties that makes it particularly suited for practical applications. Therefore, it is essential to know its fundamental enzymatic properties. Stopped-flow techniques were used to investigate the pH dependence of the reaction of SBP and hydrogen peroxide. The reaction is linearly dependent on hydrogen peroxide concentration at acidic and neutral pH with the second order rate constant k(1)=2.0x10(7) M(-1) s(-1), pH 4-8. From pH 9.3 to 10.2 the reaction is biphasic, a novel observation for a peroxidase at alkaline pH. A fast reaction has the characteristics of the reaction at neutral pH, and a slow reaction shows hyperbolic dependence on hydrogen peroxide concentration. At pH >10.5 only the slow reaction is seen. The shift in mechanism is coincident with the change in haem iron co-ordination to a six-coordinate low spin hydroxy ligated alkaline form. The pK(a) value for the alkaline transition was observed at 9.7+/-0.1, 9.6+/-0.1 and 9.9+/-0.2 by spectrophotometric titration, the fast phase amplitude, and decrease in the apparent second order rate constant, respectively. An acidic pK(a) at 3.2+/-0.3 was also determined from the apparent second order rate constant. The reactions of so...Continue Reading

References

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Mar 14, 2000·Enzyme and Microbial Technology·I Z Munir, J S Dordick
Mar 27, 2001·Protein Science : a Publication of the Protein Society·A HenriksenM Gajhede

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Citations

Sep 1, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·G BattistuzziM Sola
Aug 9, 2005·Biotechnology Letters·Kristina KnutsonArthur Ragauskas
Apr 6, 2004·Biochimica Et Biophysica Acta·Karen G Welinder, Yvonne B Larsen
Mar 27, 2001·Protein Science : a Publication of the Protein Society·A HenriksenM Gajhede
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·G V OtrokhovA I Yaropolov
Dec 27, 2005·Archives of Biochemistry and Biophysics·Liusheng Huang, Paul R Ortiz de Montellano
Jun 5, 2013·Journal of Molecular Graphics & Modelling·Vito Librando, Matteo Pappalardo
Jan 5, 2011·Journal of Inorganic Biochemistry·Tatiana MarchisEnzo Laurenti
Apr 28, 2010·Applied Microbiology and Biotechnology·Henry Joseph Oduor OgolaYoshihiro Sawa
Nov 10, 2006·Chemical Reviews·James P McEvoy, Gary W Brudvig
Jan 4, 2021·Bioprocess and Biosystems Engineering·Mengqi YeGuanjun Chen
Jul 18, 2002·Biochemistry·J K Amisha Kamal, Digambar V Behere

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