Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution.

Inorganic Chemistry
Andrew Nowakowski, David H Petering

Abstract

Zinquin (ZQ) is a commonly used sensor for cellular Zn(2+) status. It has been assumed that it measures accessible Zn(2+) concentrations in the nanomolar range. Instead, this report shows a consistent pattern across seven mammalian cell and tissue types that ZQ reacts with micromolar concentrations of Zn(2+) bound as Zn-proteins. The predominant class of products were ZQ-Zn-protein adducts that were characterized in vivo and in vitro by a fluorescence emission spectrum centered at about 470 nm, by their migration over Sephadex G-75 as protein not low molecular weight species, by the exclusion of reaction with lipid vesicles, and by their large aggregate concentration. In addition, variable, minor formation of Zn(ZQ)(2) with a fluorescence band at about 490 nm was observed in vivo in each case. Because incubation of isolated Zn-proteome with ZQ also generated similar amounts of Zn(ZQ)(2), it was concluded that this species had formed through direct ligand substitution in which ZQ had successfully competed for protein-bound Zn(2+). Parallel studies with the model Zn-proteins, alcohol dehydrogenase (ADH), and alkaline phosphatase (AP) revealed a similar picture of reactivity: ZQ(ACID) (Zinquin acid, (2-methyl-8-p-toluenesulfonamid...Continue Reading

References

May 1, 1966·Biochemistry·P PulidoB L Vallee
Jul 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P D ZalewskiI Mahadevan
Dec 3, 1999·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·H Haase, D Beyersmann
Jan 13, 2000·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·M S NasirT V O'Halloran
May 10, 2000·The Journal of Nutrition·L L PearceB R Pitt
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·A Q Truong-TranP D Zalewski
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Robert A Colvin
Jan 17, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudette M St CroixB R Pitt
Aug 30, 2002·Biochemical and Biophysical Research Communications·Hajo Haase, Detmar Beyersmann
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Daniela U SpahlKlaus-D Kröncke
Jan 7, 2006·Journal of Proteome Research·Claudia AndreiniAntonio Rosato
Aug 23, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Artur Krezel, Wolfgang Maret
Oct 24, 2007·The Journal of Nutrition·Huiyun ShenBernhard Hennig
Mar 21, 2008·Neuroscience·P PaolettiM Casado
May 16, 2008·Science Signaling·Alan R Kay, Katalin Tóth
Sep 1, 2009·Nature Methods·Jan L VinkenborgMaarten Merkx
Dec 17, 2009·Journal of Inorganic Biochemistry·Mohammad Ali NamdarghanbariDavid H Petering
Mar 5, 2010·European Journal of Immunology·Jennifer KaltenbergHajo Haase

❮ Previous
Next ❯

Citations

Feb 18, 2014·Journal of Inorganic Biochemistry·Mohammad Ali NamdarghanbariDavid H Petering
Apr 14, 2012·Metallomics : Integrated Biometal Science·Andrew Nowakowski, David Petering
Aug 14, 2013·Metallomics : Integrated Biometal Science·Anna StaszewskaWojciech Bal
Mar 5, 2014·Chemical Reviews·Kyle P CarterAmy E Palmer
Aug 8, 2014·Chemical Reviews·M Jake PushieGraham N George
Jun 3, 2015·Nature Communications·Dolores Pérez-SalaF Javier Cañada
Nov 3, 2016·Chemical Research in Toxicology·David H Petering
Dec 6, 2016·Metallomics : Integrated Biometal Science·Mohammad R Karim, David H Petering
Dec 24, 2015·Metallomics : Integrated Biometal Science·Mohammad Rezaul Karim, David H Petering
Jan 30, 2015·Chemical Communications : Chem Comm·Claudia A Blindauer
Jun 21, 2017·International Journal of Molecular Sciences·David H Petering, Afsana Mahim
Sep 13, 2018·Metallomics : Integrated Biometal Science·Angelique N BesoldValeria C Culotta
May 11, 2019·Functional Plant Biology : FPB·Ilya E ZlobinVladimir V Kuznetsov
Oct 16, 2019·Metallomics : Integrated Biometal Science·James P C CoverdaleClaudia A Blindauer
Aug 21, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Yu ZhangXuhong Qian
Nov 5, 2014·Metallomics : Integrated Biometal Science·Wolfgang Maret
Apr 2, 2014·Metallomics : Integrated Biometal Science·Andrew B NowakowskiDavid H Petering
Dec 11, 2020·Metallomics : Integrated Biometal Science·Ashley L HollingsMark J Hackett
Jul 22, 2018·Journal of the American Chemical Society·Joomyung V JunDavid M Chenoweth
Feb 27, 2013·Coordination Chemistry Reviews·Lynne M Hyman, Katherine J Franz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.