PMID: 41159Jan 1, 1978

Reactions of the sulfhydryl groups of membrane-bound bovine rhodopsin

Membrane Biochemistry
Y S Chen, W L Hubbell

Abstract

Reactions of the sulfhydryl groups of bovine rhodopsin in rod outer segment membranes have been investigated using 4,4'-dithiopyridine. This reagent is uncharged at neutral pH and rapidly equilibrates across phospholipid bilayers. Membrane-bound rhodopsin has two kinetically distinguishable sulfhydryl groups reactive to the reagent, this stoichiometry being unchanged by bleaching provided the sulfhydryl reactions themselves are carried out in the dark. The rates of the reactions, however, are substantially increased by bleaching. Irradiation of bleached membranes, either with white light or wavelengths in the neighborhood of 475 nm, results in an increase in the number of reactive sulfhydryls relative to that found for bleached membranes in the dark. A component of the light-driven reaction is dependent on the Ca2+ content of the medium.

References

Jan 1, 1976·Vision Research·J H McDowell, T P Williams
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K Hong, W L Hubbell
Aug 5, 1974·Biochemical and Biophysical Research Communications·G A RobertsonJ H Rockey
Jan 26, 1972·Nature: New Biology·H Heitzmann
May 21, 1974·Biochemistry·D S Papermaster, W J Dreyer
Dec 24, 1973·Experimental Eye Research·Y S Chen, W L Hubbell
Nov 22, 1973·Biochimica Et Biophysica Acta·W J de GripS L Bonting
Nov 22, 1973·Biochimica Et Biophysica Acta·E A Kimble, S E Ostroy
Mar 1, 1967·Archives of Biochemistry and Biophysics·D R Grassetti, J F Murray
Oct 6, 1972·Nature·H HauserM Stubbs
Nov 20, 1958·The Journal of General Physiology·R HUBBARD
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
May 1, 1952·The Journal of General Physiology·G WALD, P K BROWN

Citations

Jan 1, 1984·Vision Research·J B FindlayP Thompson
Jan 1, 1980·Neurochemistry International·F J Daemen, W J de Grip
Jun 26, 2010·Biochemistry·Arpana DuttaJudith Klein-Seetharaman
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·K CaiH Gobind Khorana
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M KimW L Hubbell
Dec 1, 1992·Photochemistry and Photobiology·Z T FarahbakhshW L Hubbell
Dec 25, 1979·Biochemistry·S P Clark, R S Molday
Mar 9, 1987·FEBS Letters·N G AbdulaevG D Chumanov
Feb 29, 2000·The Journal of Biological Chemistry·K SachsK P Hofmann

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