Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-Chaperone System

Current Protocols in Protein Science
Michal ZolkiewskiStephan N Witt

Abstract

Protein aggregation is a common problem in protein biochemistry and is linked to many cellular pathologies and human diseases. The molecular chaperone ClpB can resolubilize and reactivate aggregated proteins. This unit describes the procedure for following reactivation of an aggregated enzyme glucose-6-phosphate dehydrogenase mediated by ClpB from Escherichia coli in cooperation with another molecular chaperone, DnaK. The procedures for purification of these chaperones are also described.

References

Nov 15, 1979·Analytical Biochemistry·P A LanzettaO A Candia
Feb 1, 1975·Analytical Biochemistry·H H Hess, J E Derr
Jul 1, 1991·Journal of Bacteriology·C L SquiresC Squires
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·K MotohashiM Yoshida
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·P GoloubinoffB Bukau
May 10, 2000·The Journal of Biological Chemistry·S DiamantP Goloubinoff
Sep 13, 2000·The Journal of Biological Chemistry·M E BarnettM Zolkiewski
Dec 12, 2003·The Journal of Biological Chemistry·Valerie GrimmingerStefan Walter
Aug 4, 2005·The Journal of Biological Chemistry·Micheal E BarnettMichal Zolkiewski
Jan 26, 2006·Current Opinion in Structural Biology·Frederic RousseauLuis Serrano
Mar 28, 2006·Journal of Structural Biology·Benjamin BöslStefan Walter
May 9, 2006·Cell·Bernd BukauArthur Horwich
Apr 23, 2008·Current Protocols in Protein Science·L Zumstein
Apr 23, 2008·Current Protocols in Protein Science·P Wingfield
Apr 23, 2008·Current Protocols in Protein Science·K L Elbing, R Brent
Apr 23, 2008·Current Protocols in Protein Science·K L Elbing, R Brent
Apr 23, 2008·Current Protocols in Protein Science·C E Seidman, K Struhl
Apr 23, 2008·Current Protocols in Protein Science·Lynn A Echan, David W Speicher
Apr 23, 2008·Current Protocols in Protein Science·Bradley J S C Olson, John Markwell
Nov 15, 2008·Trends in Biochemical Sciences·Shannon M Doyle, Sue Wickner
Jan 30, 2009·Protein Science : a Publication of the Protein Society·Maria NagyMichal Zolkiewski
Dec 8, 2009·Journal of Molecular Biology·Maria NagyMichal Zolkiewski
Apr 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marika MiotSue Wickner
Jul 22, 2011·Nature·F Ulrich HartlManajit Hayer-Hartl
Aug 17, 2011·Biochimica Et Biophysica Acta·Morgan E Desantis, James Shorter
Feb 7, 2012·Archives of Biochemistry and Biophysics·Michal ZolkiewskiMaria Nagy
Apr 4, 2012·Current Protocols in Protein Science·Sean R Gallagher
Sep 13, 2013·Nature Reviews. Molecular Cell Biology·Helen Saibil
Jan 8, 2014·Disease Models & Mechanisms·Julie S Valastyan, Susan Lindquist

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Citations

Nov 26, 2019·Biochimie·Owais R HakiemJanendra K Batra
Mar 10, 2020·Journal of Molecular Biology·José Angel Fernández-HigueroJose M G Vilar

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