PMID: 6169663May 15, 1981Paper

Reactivation of Epstein-Barr virus-specific cytotoxic T cells by in vitro stimulation with the autologous lymphoblastoid cell line

International Journal of Cancer. Journal International Du Cancer
A B RickinsonM A Epstein

Abstract

Unfractionated mononuclear (UM) cells and T cells freshly prepared from the blood of adult donors were co-cultivated in microtest plate wells with progressively lower numbers of cells from the autologous EB-virus-transformed B-cell line. The fresh cells present in co-cultures from EB virus antibody-negative (seronegative) donors regularly facilitated autologous cell line outgrowth, monitored after 4 weeks, whereas outgrowth was markedly inhibited in the corresponding co-cultures from seropositive donors. Larger-scale co-cultures, set up at a ratio of 80-100 fresh UM cells to one autologous virus-transformed B cell, were harvested after 8 to 12 days and the T-cell subpopulation was examined for cytotoxicity both by growth inhibition and by chromium release assays. Cytotoxic T cells were generated exclusively in seropositive donor co-cultures and were strongly active against the autologous virus-transformed cell line without affecting either autologous uninfected B cells or any of a range of EB virus genome-negative target cell lines chosen as sensitive indicators of non-specific cytotoxicity. Recognition of allogeneic EB-virus-transformed cells was restricted to those whose HLA-A and/or B and/or B and/or C antigen expression mat...Continue Reading

References

Jan 1, 1979·Immunological Reviews·F M BrodskyW F Bodmer
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Svedmyr, M Jondal
Jan 1, 1977·Annual Review of Microbiology·M A Epstein, B G Achong
May 15, 1979·International Journal of Cancer. Journal International Du Cancer·A B RickinsonJ H Pope
May 15, 1979·International Journal of Cancer. Journal International Du Cancer·D J MossJ H Pope
Jun 1, 1978·The Journal of Experimental Medicine·M Jondal, S Targan
Jan 1, 1979·Journal of Immunological Methods·R van LambalgenB A Bradley
Jul 1, 1977·Vox Sanguinis·F Gmelig-Meyling, R E Ballieux
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D J Charron, H O McDevitt
Nov 15, 1971·International Journal of Cancer. Journal International Du Cancer·K NilssonG Henle
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G KleinC Sundström
Jun 15, 1974·International Journal of Cancer. Journal International Du Cancer·E A SvedmyrG Klein
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·G HenleV Diehl
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·J R NorthM A Epstein
Jan 15, 1980·International Journal of Cancer. Journal International Du Cancer·A B RickinsonN Ahlberg
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·I S MiskoJ H Pope

❮ Previous
Next ❯

Citations

Jan 1, 1984·Immunogenetics·E GomardJ P Lévy
Jan 1, 1989·Research in Immunology·R A Koup, J L Sullivan
Nov 25, 2000·Molecular Pathology : MP·K J Flavell, P G Murray
Apr 1, 1990·Irish Journal of Medical Science·M J Turner
Jan 1, 1982·Rheumatology International·J S GastonM A Epstein
Jul 1, 1983·Journal of Clinical Immunology·D T Purtilo, J Linder
Jul 1, 1983·Journal of Clinical Immunology·J F GreallyD Purtilo
Jan 1, 1983·Journal of Cancer Research and Clinical Oncology·J Zeuthen
Aug 12, 1993·Annals of the New York Academy of Sciences·M G MasucciR Dolcetti
Sep 1, 1984·Clinical Immunology and Immunopathology·I S MiskoJ H Pope
Aug 15, 1987·International Journal of Cancer. Journal International Du Cancer·M T BejaranoG Klein
Sep 15, 1988·International Journal of Cancer. Journal International Du Cancer·M T BejaranoE Klein
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jill M BrooksAlan B Rickinson
Apr 20, 2017·Journal of Oncology·Shyam Singh, Hem Chandra Jha
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C IbischH Vié
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D F DukersJ M Middeldorp
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Igor J KoralnikNorman L Letvin
Apr 1, 1993·Clinical and Experimental Immunology·K C WatretA G Bird

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.