PMID: 9545552Apr 18, 1998Paper

Reactive iron species in biological fluids activate the iron-sulphur cluster of aconitase

Biochimica Et Biophysica Acta
S MumbyJ M Gutteridge

Abstract

Low molecular mass iron (LMrFe) can appear in plasma when the transferrin becomes fully iron loaded. Such iron poses a risk factor for oxidative damage, and for microbial virulence. A previous novel approach to the detection and measurement of LMrFe in plasma was the use of the iron-binding properties of the glycopeptide antitumour antibiotic bleomycin and its ability to degrade DNA in the presence of oxygen, bound iron, and an iron reducing agent. Since bleomycin is a non-physiological ligand with iron-binding and redox cycling properties, it has been suggested that it may not be a valid biological model for detecting and measuring LMrFe. To address these concerns we have developed a biological approach to the detection and measurement of LMrFe based on the activation of iron-requiring aconitase. Parallel measurements, in a variety of clinical conditions in which there was a complete saturation of the plasma transferrin, showed that the bleomycin assay and the aconitase assay can give similar results for LMrFe.

References

Jul 27, 1992·FEBS Letters·B Halliwell, J M Gutteridge
Jan 1, 1991·Free Radical Biology & Medicine·J M Gutteridge
Jan 1, 1986·Free Radical Research Communications·J M Gutteridge, Y Y Hou
Oct 1, 1981·The Biochemical Journal·J M GutteridgeB Halliwell
Jan 1, 1994·Abdominal Imaging·M T WuC W Wu
Dec 24, 1996·Biochemical and Biophysical Research Communications·J M GutteridgeN Taniguchi

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Citations

Aug 7, 2008·PloS One·Jinze XuChristiaan Leeuwenburgh
Jul 30, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Satoshi ItoYutaka Kohgo

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