Reactive oxidants mediate TNF-alpha-induced leukocyte adhesion to rat mesenteric venular endothelium

The American Journal of Physiology
Y MoritaG B Bulkley

Abstract

We investigated the role of reactive oxygen metabolites (ROMs) as potential mediators of tumor necrosis factor-alpha (TNF-alpha)-stimulated neutrophil adhesion to rat mesenteric venules in vivo, using intravital microscopy and fixed whole mount preparations of mesentery. Intraperitoneal injection of TNF-alpha significantly increased leukocyte rolling, adhesion, and emigration in a dose- and time-dependent manner. Leukocyte adhesion and emigration, but not rolling, were significantly attenuated by prior intravenous administration of monoclonal anti-intercellular adhesion molecule-1 (ICAM-1). Rolling leukocyte flux was significantly attenuated by intravenous preadministration of superoxide dismutase (SOD), catalase, or both. Only catalase or SOD plus catalase significantly inhibited leukocyte adhesion. Catalase alone inhibited emigration. Moreover, postadhesive treatment with catalase but not SOD, 4 h after TNF-alpha administration reduced the flux of rolling (but not adherent) leukocytes that had previously increased in response to TNF-alpha. Intragastric allopurinol (50 mg/kg at 3 and 18 h before TNF-alpha administration) or 3 wk of a tungsten-enriched diet substantially inhibited xanthine oxidase activity but had no significan...Continue Reading

Citations

Mar 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Dhananjay K KaulRobert P Hebbel
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·C B Henry, B R Duling
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Ann L BaldwinAbdu I Alayash

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