Reactive oxygen species are involved in shear stress-induced intercellular adhesion molecule-1 expression in endothelial cells

Arteriosclerosis, Thrombosis, and Vascular Biology
J J ChiuD L Wang

Abstract

Vascular endothelial cells (ECs) are constantly subjected to flow-induced shear stress. Although the effects of shear stress on ECs are well known, the intracellular signal mechanisms remain largely unclear. Reactive oxygen species (ROS) have recently been suggested to act as intracellular second messengers. The potential role of ROS in shear-induced gene expression was examined in the present study by subjecting ECs to a shear force using a parallel-plate flow chamber system. ECs under shear flow increased their intracellular ROS as indicated by superoxide production. This superoxide production was maintained at an elevated level as shear flow remained. Sheared ECs, similar to TNF(alpha)-, PMA-, or H2O2-treated cells, increased their intercellular adhesion molecule-1 (ICAM-1) mRNA levels in a time-dependent manner. Pretreatment of ECs with an antioxidant, N-acetyl-cysteine (NAC) or catalase, inhibited this shear-induced or oxidant-induced ICAM-1 expression. ROS that were involved in the shear-induced ICAM-1 gene expression were further substantiated by functional analysis using a chimera containing the ICAM-1 promoter region (-850 bp) and the reporter gene luciferase. Shear-induced promoter activities were attenuated by pretre...Continue Reading

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K NakazonoM Inoue
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F J StaalL A Herzenberg
Jul 16, 1990·Biochemical and Biophysical Research Communications·M U NollertL V McIntire
Mar 12, 1987·Journal of Immunological Methods·H Gyllenhammar
Aug 1, 1995·The American Journal of Physiology·G HelmlingerR M Nerem
May 28, 1993·Biochemical and Biophysical Research Communications·A OhtsukaM Miyasaka
Jan 26, 1995·Biochemical and Biophysical Research Communications·H TsuboiA Kamiya
Sep 1, 1994·The American Journal of Physiology·M J KuchanJ A Frangos
Jun 15, 1994·Biochemical and Biophysical Research Communications·Q LanP F Davies
Nov 1, 1993·Journal of Biomechanical Engineering·R M Nerem
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N ResnickM A Gimbrone
Feb 23, 1996·The Journal of Biological Chemistry·K Z GuytonN J Holbrook
Jun 28, 1996·The Journal of Biological Chemistry·Y Y LoT F Cruz
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·B V KhanR M Medford
Nov 1, 1996·Molecular and Cellular Biology·Y S LiS Chien

❮ Previous
Next ❯

Citations

Aug 10, 2006·Journal of Mathematical Biology·Wensheng Zhang, Aurélie Edwards
Mar 7, 2007·Annals of Biomedical Engineering·Charles I JonesB Rita Alevriadou
May 4, 2000·The American Journal of Pathology·J H YangR T Lee
Nov 22, 2001·European Journal of Pharmacology·J CortijoE J Morcillo
Nov 15, 2003·Journal of Biomechanics·Jeng-Jiann ChiuShunichi Usami
Oct 13, 2000·British Journal of Pharmacology·G NickenigM Böhm
Sep 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qian ZhangMariusz A Wasik
Sep 22, 2006·Antioxidants & Redox Signaling·Hanjoong JoAmy Mowbray
Sep 24, 2008·Antioxidants & Redox Signaling·Arshad Rahman, Fabeha Fazal
Dec 22, 2010·Tissue Engineering. Part C, Methods·Bertrand DavidHervé Petite
Jun 28, 2003·Transplantation·Vassilios S AvlonitisJohn H Dark
Jan 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Zhaosheng HanB Rita Alevriadou
Aug 3, 2006·American Journal of Veterinary Research·Mark A Oyama, Sridar V Chittur
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Filomena de NigrisClaudio Napoli
Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Filomena de NigrisClaudio Napoli
Nov 15, 2011·Annual Review of Physiology·Elizabeth A BrowningAron B Fisher
Aug 31, 2002·Endothelium : Journal of Endothelial Cell Research·Shailesh Y DesaiDamir Janigro
Feb 8, 2003·Endothelium : Journal of Endothelial Cell Research·James R Stone, Tucker Collins
Dec 10, 2015·Antioxidants & Redox Signaling·Joan Fernández EsmeratsHanjoong Jo
Dec 31, 2015·Antioxidants & Redox Signaling·Arif Yurdagul, A Wayne Orr
Sep 10, 2014·Small GTPases·Caitlin Collins, Ellie Tzima
Apr 15, 2017·Journal of Comparative Pathology·G MarkbyB M Corcoran
Apr 14, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S WeissJ Pratschke
Feb 28, 2009·Journal of Cardiovascular Pharmacology and Therapeutics·Kailash Prasad
Aug 13, 2004·Vascular and Endovascular Surgery·Joseph L UnthankMichael C Dalsing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.