Reactive oxygen species are involved in the IFN-γ-stimulated production of Th2 chemokines in HaCaT keratinocytes

Journal of Cellular Physiology
Xu-Feng QiKyu-Jae Lee

Abstract

The increased generation of reactive oxygen species (ROS) induces inflammation in different cell types. However, it is unclear whether ROS play an essential role in the production of thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22) in keratinocytes. Here, we investigated the function of ROS in the production of these two Th2 chemokines in interferon-gamma (IFN-γ)-treated HaCaT keratinocytes. We found that IFN-γ-induced production of both chemokines in parallel with the increased generation of intracellular ROS. A ROS scavenger, N-acetyl cysteine (NAC), significantly inhibited the IFN-γ-induced production of chemokines as well as the activation of I kappa-B (IκB)-nuclear factor-kappa B (NF-κB). Inhibitors of Janus family kinases (JAKs), p38 mitogen-activated kinase (MAPK), and NF-κB suppressed IFN-γ-induced production of TARC and MDC. NF-κB activation was inhibited by both inhibitors of JAKs and p38 MAPK. Importantly, IFN-γ-stimulated phosphorylation of p38 MAPK was significantly suppressed by JAKs inhibitors, but not significantly affected by NAC or L-buthionine sulfoximine (L-BSO). However, IFN-γ-stimulated activation of IκB and NF-κB was suppressed by NAC but enhanced by BSO....Continue Reading

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Citations

Oct 19, 2013·European Journal of Immunology·Theresa GlobischIrmgard Förster
Jan 10, 2012·Biochemical and Biophysical Research Communications·Dong-Joo KwonJinseu Park
Jul 29, 2017·PloS One·Asmae BelhajBenoît Rondelet
Feb 6, 2017·The Journal of Biological Chemistry·Elyse A SchmidtGregory A Taylor
Sep 16, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·You Ah KimByoung Jun Park
May 13, 2019·Molecular and Cellular Biochemistry·Kailash Prasad
May 30, 2017·Oxidative Medicine and Cellular Longevity·Aijun SunChuansen Zhang

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