Reactive oxygen species differentially affect T cell receptor-signaling pathways

The Journal of Biological Chemistry
Saso CemerskiPaola Romagnoli

Abstract

Oxidative stress plays an important role in the induction of T lymphocyte hyporesponsiveness observed in several human pathologies including cancer, rheumatoid arthritis, leprosy, and AIDS. To investigate the molecular basis of oxidative stress-induced T cell hyporesponsiveness, we have developed an in vitro system in which T lymphocytes are rendered hyporesponsive by co-culture with oxygen radical-producing activated neutrophils. We have observed a direct correlation between the level of T cell hyporesponsiveness induced and the concentration of reactive oxygen species produced. Moreover, induction of T cell hyporesponsiveness is blocked by addition of N-acetyl cysteine, Mn(III)tetrakis(4-benzoic acid)porphyrin chloride, and catalase, confirming the critical role of oxidative stress in this system. The pattern of tyrosine-phosphorylated proteins was profoundly altered in hyporesponsive as compared with normal T cells. In hyporesponsive T cells, T cell receptor (TCR) ligation no longer induced phospholipase C-gamma1 activation and caused reduced Ca(2+) flux. In contrast, despite increased levels of ERK1/2 phosphorylation, TCR-dependent activation of mitogen-activated protein kinase ERK1/2 was unaltered in hyporesponsive T lymph...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S B KannerJ A Ledbetter
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F J StaalL A Herzenberg
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S Hardwick, B M Sefton
Jun 1, 1995·European Journal of Immunology·M E AllenP A Bacon
May 1, 1995·Free Radical Research·T M Buttke, P A Sandstrom
Sep 8, 1995·The Journal of Biological Chemistry·H M LanderA Novogrodsky
May 1, 1995·The Journal of Experimental Medicine·T AoeT Saito
Sep 1, 1996·The Biochemical Journal·M SundaresanT Finkel
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·M OtsujiT Saito
Jan 1, 1997·Annual Review of Immunology·H NakamuraJ Yodoi
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S I GringhuisC L Verweij
Mar 4, 2000·Free Radical Biology & Medicine·R G Allen, M Tresini
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S P HehnerW Dröge
May 15, 2001·The Journal of Biological Chemistry·X T WangN J Holbrook
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Giovanna AngeliniAnna Rubartelli
Aug 1, 1991·Trends in Cell Biology·R Schreck, P A Baeuerle

❮ Previous
Next ❯

Citations

Aug 26, 2003·Journal of Autoimmunity·Rikard Holmdahl
Nov 18, 2006·Epidemiology and Infection·J UberosA Muñoz-Hoyos
Jul 15, 2005·European Journal of Clinical Nutrition·R VijayaraghavanC Panneerselvam
Sep 4, 2010·Antioxidants & Redox Signaling·Subramaniam Ponnappan, Usha Ponnappan
Jan 26, 2007·Proceedings. Biological Sciences·Carlos Alonso-AlvarezGabriele Sorci
Jan 26, 2005·American Journal of Nephrology·E Nigel Wardle
Aug 10, 2005·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Elizaveta Fasler-KanPeter Meyer
Sep 3, 2008·Expert Review of Proteomics·Steve Wilson, Dawn J Mazzatti
May 4, 2010·Cellular Immunology·David KoosEwa Carrier
Jul 28, 2009·Mechanisms of Ageing and Development·Thorsten PetersKarin Scharffetter-Kochanek
Jun 15, 2007·Immunology Letters·György NagyAndrew P Cope
Jul 3, 2007·Experimental Gerontology·Anis LarbiGraham Pawelec
Feb 14, 2004·Immunological Reviews·Jeffrey C Rathmell
Aug 26, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Lorenzo Pérez-Rodríguez
Apr 22, 2011·Journal of Cellular and Molecular Medicine·Swati PatelShubhada Chiplunkar
Nov 19, 2010·BJOG : an International Journal of Obstetrics and Gynaecology·Jennifer Mier-CabreraCésar Hernández-Guerrero
Jan 18, 2014·European Journal of Immunology·Shirin Kalyan, Dieter Kabelitz
Oct 17, 2015·Journal of Biomedical Science·Aleksey V BelikovLuca Simeoni
Sep 29, 2004·Free Radical Biology & Medicine·Mark S Williams, Jaeyul Kwon
Jun 19, 2004·Drug Discovery Today·Brian J Day
May 27, 2005·Free Radical Biology & Medicine·Oswaldo G Rodriguez-MoraRichard A Franklin
Apr 23, 2013·Mediators of Inflammation·Stella VodoAnna Maria Aloisi
Dec 15, 2010·International Journal of Radiation Biology·Asma RizviDaila S Gridley
Aug 23, 2016·Oxidative Medicine and Cellular Longevity·Xinfeng ChenYi Zhang
Nov 10, 2017·Antioxidants & Redox Signaling·Carlota RecioDavid R Greaves
Aug 22, 2015·Journal of the American Society of Nephrology : JASN·Sanjeev NoelHamid Rabb
Mar 23, 2019·Blood·Morgan A GieseAnna Huttenlocher
Aug 8, 2019·Archives of Physiology and Biochemistry·Taofeek O Usman, Lawrence A Olatunji
Aug 21, 2019·Cancers·Frank WeinbergDeepak Nagrath

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.