PMID: 9187701Jun 1, 1997Paper

Reactive oxygen species generation by seminal cells during cryopreservation

Urology
A W WangK R Loughlin

Abstract

To test the hypothesis that conventionally used procedures for semen cryopreservation may cause an increase in the production of reactive oxygen species (ROS) by sperm or by seminal leukocytes, which may contribute to poor sperm function following cryopreservation. Eighteen semen specimens with normal parameters from healthy male donors 22 to 40 years of age were each divided into two portions. The first portion was combined 1:1 with Test Yolk Buffer-Glycerol Freezing Medium and was frozen by gradual cooling into liquid nitrogen (-196 degrees C). The second portion was washed and the cells were resuspended in Sperm Washing Medium (SWM) and incubated at room temperature to serve as controls. After a period of treatment, frozen samples were thawed and semen cells were washed and resuspended in SWM. ROS generation by semen cells from each treatment group was measured on a luminometer. Sperm motility, sperm viability, and sperm membrane integrity were also measured in both control and freeze-thaw samples. To further assess ROS generation by semen cells during the cooling process, aliquots of washed semen cells and purified polymorphonuclear leukocytes (PMNs) were incubated separately at different temperature conditions (37 degrees ...Continue Reading

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Citations

Jul 29, 2004·Journal of Assisted Reproduction and Genetics·Madeleine CounselPeter Burton
Oct 23, 2009·Biology of Reproduction·Megan J McCarthyStuart A Meyers
Jun 23, 2012·PloS One·Mary HagedornStuart A Meyers
Jun 13, 2001·Biochemical and Biophysical Research Communications·S HondaJ T Handa
Mar 14, 2014·Biopreservation and Biobanking·A BissoyiSunil K Sarangi
Feb 23, 2010·Systems Biology in Reproductive Medicine·Jaime GosálvezRocio Nuñez
Jul 4, 2012·Reproductive Toxicology·G LavranosR Angelopoulou
Aug 31, 2010·Reproductive Biomedicine Online·Tamer M SaidAshok Agarwal
May 19, 2010·Animal Reproduction Science·Jian-Hong HuYa-Ping Xin
Feb 12, 2005·BJU International·Ashok Agarwal, Tamer M Said
Jun 11, 2005·Molecular Reproduction and Development·D M NeildB M Gadella
Apr 15, 2009·Reproduction in Domestic Animals = Zuchthygiene·J PonthierS Deleuze
Feb 2, 2010·Reproduction in Domestic Animals = Zuchthygiene·J-H HuQ-W Li
Oct 8, 2005·Fertility and Sterility·Ekaterina V ZubkovaBernard Robaire
Jun 29, 2014·Research in Veterinary Science·A A CarvalhoA P R Rodrigues
Aug 8, 2015·Journal of Assisted Reproduction and Genetics·Lara MoutthamPierre Comizzoli
Apr 8, 2014·Systems Biology in Reproductive Medicine·Zahra BateniMohammad Hossein Nasr-Esfahani
Jun 18, 2015·Zygote : the Biology of Gametes and Early Embryos·Wei ZhangWenqin Lin

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