Reactive oxygen species impair the excitation-contraction coupling of papillary muscles after acute exposure to a high copper concentration

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Filipe Martinuzo FilettiM R Simões

Abstract

Copper is an essential metal for homeostasis and the functioning of living organisms. We investigated the effects of a high copper concentration on the myocardial mechanics, investigating the reactive oxygen species (ROS) mediated effects. The developed force of papillary muscles was reduced after acute exposure to a high copper concentration and was prevented by co-incubation with tempol, DMSO and catalase. The reuptake of calcium by the sarcoplasmic reticulum was reduced by copper and restored by tempol. The contractile response to Ca2+ was reduced and reversed by antioxidants. The response to the β-adrenergic agonist decreased after exposure to copper and was restored by tempol and catalase. In addition, the in situ detection showed increased O2·- and OH·. Contractions dependent on the sarcolemmal Ca2+ influx were impaired by copper and restored by antioxidants. Myosin-ATPase activity decreased significantly after copper exposure. In conclusion, a high copper concentration can acutely impair myocardial excitation-contraction coupling, reduce the capacity to generate force, reduce the Ca2+ inflow and its reuptake, and reduce myosin-ATPase activity, and these effects are mediated by the local production of O2·-, OH· and H2O2. ...Continue Reading

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Citations

Jan 16, 2019·Biological Trace Element Research·Aleksandra WilkElżbieta Kalisińska
Aug 23, 2020·International Journal of Molecular Sciences·Jarosław NuszkiewiczKarolina Szewczyk-Golec

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